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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Fluorescence study of protein-lipid complexes with a new symmetric squarylium probe.
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Fluorescence study of protein-lipid complexes with a new symmetric squarylium probe.

机译：新型对称方酸探针对蛋白质-脂质复合物的荧光研究。

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摘要

The novel symmetric squarylium derivative SQ-1 has been synthesized and tested for its sensitivity to the formation of protein-lipid complexes. SQ-1 binding to the model membranes composed of zwitterionic lipid phosphatidylcholine (PC) and its mixtures with anionic lipid cardiolipin (CL) in different molar ratios was found to be controlled mainly by hydrophobic interactions. Lysozyme (Lz) and ribonuclease A (RNase) exerted an influence on the probe association with lipid vesicles resulting presumably from the competition between SQ-1 and the proteins for bilayer free volume and modification of its properties. The magnitude of this effect was much higher for lysozyme which may stem from the amphipathy of protein alpha-helix involved in the membrane binding. Varying membrane composition provides evidence for the dye sensitivity to both hydrophobic and electrostatic protein-lipid interactions. Fluorescence anisotropy studies uncovered the restriction of SQ-1 rotational mobility in lipid environment in thepresence of Lz and RNase being indicative of the incorporation of the proteins into bilayer interior. The results of binding, fluorescence quenching and kinetic experiments suggested lysozyme-induced local lipid demixing upon protein association with negatively charged membranes with threshold concentration of CL for the lipid demixing being 10 mol%.
机译：已经合成了新型对称方酸衍生物SQ-1，并测试了其对蛋白质-脂质复合物形成的敏感性。发现SQ-1以不同摩尔比结合由两性离子脂质磷脂酰胆碱（PC）及其混合物与阴离子脂质心磷脂（CL）组成的模型膜，主要受疏水相互作用控制。溶菌酶（Lz）和核糖核酸酶A（RNase）对与脂质囊泡的探针缔合有影响，这可能是由于SQ-1与蛋白质之间的双层自由体积竞争和其性质改变而引起的。对于溶菌酶，这种作用的强度要高得多，这可能是由于参与膜结合的蛋白质α-螺旋的两亲性引起的。膜组成的变化为染料对疏水和静电蛋白质-脂质相互作用的敏感性提供了证据。荧光各向异性研究揭示了脂质环境中Sq-1旋转运动的限制，因为Lz和RNase的存在表明蛋白质掺入双层内部。结合，荧光猝灭和动力学实验的结果表明，当蛋白质与带负电荷的膜结合时，溶菌酶诱导的局部脂质分解，其中脂质分解的CL的阈值浓度为10mol％。

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