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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Expression and function of the costimulatory molecules B7-1 (CD80) and B7-2 (CD86) in an in vitro model of the human blood--brain barrier.
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Expression and function of the costimulatory molecules B7-1 (CD80) and B7-2 (CD86) in an in vitro model of the human blood--brain barrier.

机译：共刺激分子B7-1（CD80）和B7-2（CD86）在人血脑屏障体外模型中的表达和功能。

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摘要

The interaction of B7 molecules with their ligand provides important accessory signals for optimal T cell activation and proliferation. In this study the in vitro expression of B7-1 and B7-2 by human brain microvessel endothelial cells (HBMEC) was investigated by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry. In addition, the contribution of B7 molecules to T cell proliferation on cerebral endothelial cells was studied by coincubating purified CD4+ T cells with resting or cytokine activated HBMEC. Untreated cultures constitutively expressed B7-2 RNA and surface protein, but lacked B7-1 expression. Treatment with TNF-alpha and IFN-gamma upregulated B7-2 and induced de novo expression of B7-1. Monoclonal blocking antibodies to B7-1 or B7-2 and human CTLA-4Ig chimeric protein significantly reduced the ability of HBMEC to support alpha-CD3-induced proliferation of CD4+ T lymphocytes. Expression of B7 glycoproteins and the ability to provide secondary signals for T cell proliferation suggest a potential role of the human cerebral endothelium in T cell activation during the early stages of central nervous system inflammation.
机译：B7分子与其配体的相互作用为优化T细胞活化和增殖提供了重要的辅助信号。在这项研究中，通过半定量逆转录酶-聚合酶链反应（RT-PCR）和免疫细胞化学研究了人脑微血管内皮细胞（HBMEC）B7-1和B7-2的体外表达。另外，通过将纯化的CD4 + T细胞与静止或细胞因子激活的HBMEC共孵育，研究了B7分子对T细胞在脑内皮细胞增殖中的贡献。未经处理的培养物组成性表达B7-2 RNA和表面蛋白，但缺乏B7-1表达。 TNF-α和IFN-γ处理可上调B7-2并诱导B7-1从头表达。针对B7-1或B7-2和人CTLA-4Ig嵌合蛋白的单克隆阻断抗体显着降低了HBMEC支持α-CD3诱导的CD4 + T淋巴细胞增殖的能力。 B7糖蛋白的表达以及为T细胞增殖提供次级信号的能力表明，人脑内皮在中枢神经系统炎症的早期阶段在T细胞活化中具有潜在作用。

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