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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Antigen recognition properties of a Vgamma1.3Vdelta2-T-cell receptor from a rare variant of polymyositis.
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Antigen recognition properties of a Vgamma1.3Vdelta2-T-cell receptor from a rare variant of polymyositis.

机译：来自多发性肌炎的罕见变体的Vgamma1.3Vdelta2-T细胞受体的抗原识别特性。

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摘要

Previously we partially characterized an autoreactive human Vgamma1.3Vdelta2-T-cell receptor (TCR) that had originally been identified in muscle of a patient with an unusual form of polymyositis. This TCR recognizes a muscle-associated auto-antigen in a CDR3-dependent, MHC non-restricted way. Here we show that this TCR also recognizes an antigen from Escherichia coli. Like the muscle-associated mammalian antigen, the bacterial antigen is recognized in a CDR3-dependent, but MHC-non-restricted way. Both antigens have strikingly similar molecular characteristics suggesting that their epitopes are at least very similar. The dissociation kinetics of the bacterial antigen-TCR complexes was investigated by surface plasmon resonance using soluble single-chain TCR molecules produced in COS-7 cells. The measured dissociation rate constant (k(off)=5.7x10(-3) s(-1)) shows that the complexes dissociate more slowly than most previously described antigen/alphabeta-TCR complexes, but much faster than antibody/antigen pairs. These results (a) provide further insight into the molecular properties of this unusual TCR, and (b) should help in future attempts to identify the elusive target antigen(s).
机译：以前，我们部分表征了一种自身反应性的人Vgamma1.3Vdelta2-T细胞受体（TCR），该受体最初在患有异常多型肌炎的患者的肌肉中被发现。该TCR以依赖CDR3的MHC非限制性方式识别与肌肉相关的自身抗原。在这里，我们显示该TCR还识别大肠杆菌的抗原。像肌肉相关的哺乳动物抗原一样，细菌抗原以依赖CDR3的方式识别，但不受MHC的限制。两种抗原具有惊人相似的分子特征，表明它们的表位至少非常相似。使用在COS-7细胞中产生的可溶性单链TCR分子，通过表面等离子体共振研究了细菌抗原-TCR复合物的解离动力学。测得的解离速率常数（k（off）= 5.7x10（-3）s（-1））显示，与大多数先前描述的抗原/ alphabeta-TCR复合物相比，复合物的解离速度较慢，但​​比抗体/抗原对的分解速度要快得多。这些结果（a）提供了对该不寻常TCR分子特性的进一步了解，并且（b）应该有助于将来尝试鉴定难以捉摸的靶抗原。

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