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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Age-dependent changes in the expression of dopamine receptor subtypes in human peripheral blood lymphocytes.
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Age-dependent changes in the expression of dopamine receptor subtypes in human peripheral blood lymphocytes.

机译：人外周血淋巴细胞中多巴胺受体亚型表达的年龄依赖性变化。

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摘要

The pharmacological profile and the density of dopamine D3 and D5 receptor subtypes expressed by human peripheral blood lymphocytes of subjects of different ages (ranging from 20 to 75 years) were assessed using radioligand binding techniques. Dopamine D3 receptor was assayed with [3H]7-hydroxy-N,N-di-n-propyl-2-aminotetraline ([3H]7-OH-DPAT) as a ligand. Dopamine D5 receptor was assayed using [3HIR]-(+)-(-chloro-2,3,4,5, tetrahydro-5-phenyl-1H-3-benzazepin-al-hemimaleate) ([3H]SCH 23390) as a ligand. The affinity and the pharmacological profile of [3H]7-OH-DPAT and [3H]SCH 23390 at dopamine D3 and D5 receptor, respectively, were similar in subjects of different ages. The density of dopamine D3 receptor binding sites was slightly decreased in subjects of 30-39 years in comparison with younger individuals. A remarkable loss of dopamine D3 receptor was then found between 40 and 49 years of age in comparison with younger subjects. A further slight decrease was noticeable between 50 and 59 years of age. The number of [3H]7-OH-DPAT binding sites was then stabilized after 60 years of age. The density of dopamine D5 receptor binding sites did not show age-dependent changes. The above findings indicate the occurrence of a decline in the density of lymphocyte dopamine D3 but not D5 receptor between adult and mature subjects. The possibility that dopamine D3 receptor assay in peripheral blood lymphocytes may represent a tool for investigating dopamine receptor function in aging and age-related neurological disorders is discussed.
机译：使用放射性配体结合技术评估了不同年龄（20至75岁）受试者的人外周血淋巴细胞表达的多巴胺D3和D5受体亚型的药理特性和密度。用[3H] 7-羟基-N，N-二-正丙基-2-氨基四氢萘（[3H] 7-OH-DPAT）作为配体测定多巴胺D3受体。多巴胺D5受体使用[3HIR]-（+）-（-chloro-2,3,4,5，四氢-5-苯基-1H-3-benzazepin-al-hemimaleate）（[3H] SCH 23390）进行测定配体。 [3H] 7-OH-DPAT和[3H] SCH 23390在多巴胺D3和D5受体上的亲和力和药理学特征在不同年龄的受试者中相似。与年轻个体相比，在30-39岁的受试者中，多巴胺D3受体结合位点的密度略有降低。与年轻受试者相比，在40至49岁之间发现了多巴胺D3受体的明显丧失。在50至59岁之间，还有进一步的轻微下降。 60岁后，[3H] 7-OH-DPAT结合位点的数目稳定下来。多巴胺D5受体结合位点的密度没有显示出年龄依赖性的变化。上述发现表明在成年和成年受试者之间发生了淋巴细胞多巴胺D3而不是D5受体密度下降的情况。讨论了外周血淋巴细胞中多巴胺D3受体测定可能代表研究多巴胺受体在衰老和与年龄相关的神经系统疾病中的功能的可能性。

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