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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Differential expression of interferon-gamma receptor on human glial cells in vivo and in vitro.
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Differential expression of interferon-gamma receptor on human glial cells in vivo and in vitro.

机译：体内和体外干扰素-γ受体在人神经胶质细胞上的差异表达。

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摘要

Although significant effects of interferon-gamma (IFN-gamma) on glial cells are well documented, information on the expression level and localization of glial IFN-gamma receptors (IFN-gamma-R) in the human central nervous system (CNS) is sparse. To examine the glial expression of IFN-gamma-R in the human CNS, immunohistochemistry and quantitative analyses were performed on Alzheimer disease hippocampus, Parkinson disease substantia nigra, amyotrophic lateral sclerosis spinal cord and corresponding areas from non-neurological cases. Almost all IFN-gamma-R-positive (IFN-gamma-R(+)) cells corresponded to GFAP-positive (GFAP(+)) astrocytes, while none of IFN-gamma-R(+) cells corresponded to IBA1-positive (IBA1(+)) microglia or MBP-positive (MBP(+)) oligodendrocytes in these neurological cases. We observed a similar pattern of glial IFN-gamma-R expression in non-neurological cases. Also, we quantitatively analyzed the IFN-gamma-R expression by cultured human glial cells using immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). In contrast to in vivo results, almost all IFN-gamma-R(+) cells were IBA1(+) in microglial cultures, GFAP(+) in astrocytic cultures and MBP(+) in oligodendrocytic cultures. Moreover, no significant difference in IFN-gamma-R mRNA expression was found for these glial cell types by RT-PCR. These results suggest that the microglial and oligodendrocytic expression levels of IFN-gamma-R are much lower than the astrocytic expression levels in the human CNS in vivo, whereas all three types of glial cells constitutively express IFN-gamma-R when cultured in vitro.
机译：尽管干扰素-γ（IFN-γ）对神经胶质细胞的显著作用已得到充分证明，但有关人中枢神经系统（CNS）中神经胶质IFN-γ受体（IFN-γ-R）的表达水平和定位的信息仍然很少。为了检查IFN-γ-R在人中枢神经系统中的神经胶质表达，对阿尔茨海默氏病海马，帕金森病黑质，肌萎缩性脊髓侧索硬化症脊髓和非神经病学病例的相应区域进行了免疫组织化学和定量分析。几乎所有的IFN-γ-R（+）细胞都对应于GFAP阳性（GFAP（+））星形胶质细胞，而没有一个IFN-γ-R（+）细胞对应于IBA1阳性。 （IBA1（+））小胶质细胞或MBP阳性（MBP（+））少突胶质细胞。我们在非神经系统病例中观察到了类似的神经胶质IFN-γ-R表达模式。另外，我们使用免疫细胞化学和逆转录聚合酶链反应（RT-PCR）定量分析了培养的人胶质细胞的IFN-γ-R表达。与体内结果相反，几乎所有的IFN-γ-R（+）细胞在小胶质细胞培养物中都是IBA1（+），在星形胶质细胞培养物中是GFAP（+），在少突胶质细胞培养物中是MBP（+）。此外，通过RT-PCR未发现这些神经胶质细胞类型的IFN-γ-RmRNA表达有显着差异。这些结果表明，IFN-γ-R的小胶质细胞和少突胶质细胞表达水平远低于人CNS体内的星形胶质细胞表达水平，而当体外培养时，所有三种类型的神经胶质细胞都组成性表达IFN-γ-R。

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