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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Pro-inflammatory T-lymphocytes rapidly infiltrate into the brain and contribute to neuronal injury following cardiac arrest and cardiopulmonary resuscitation
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Pro-inflammatory T-lymphocytes rapidly infiltrate into the brain and contribute to neuronal injury following cardiac arrest and cardiopulmonary resuscitation

机译：促炎性T淋巴细胞迅速渗入大脑，并在心脏骤停和心肺复苏后导致神经元损伤

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Although inflammatory mechanisms have been linked to neuronal injury following global cerebral ischemia, the presence of infiltrating peripheral immune cells remains understudied. We performed flow cytometry of single cell suspensions obtained from the brains of mice at varying time points after global cerebral ischemia induced by cardiac arrest and cardiopulmonary resuscitation (CA/CPR) to characterize the influx of lymphocytes into the injured brain. We observed that CA/CPR caused a large influx of lymphocytes within 3h of resuscitation that was maintained for the 3day duration of our experiments. Using cell staining flow cytometry we observed that the large majority of infiltrating lymphocytes were CD4+ T cells. Intracellular stains revealed a large proportion of pro-inflammatory T cells expressing either TNFα or INFγ. Importantly, the lack of functional T cells in TCRα knockout mice reduced neuronal injury following CA/CPR, implicating pro-inflammatory T cells in the progression of ischemic neuronal injury. Finally, we made the remarkable observation that the novel CD4+CD40+ (Th40) population of pro-inflammatory T cells that are strongly associated with autoimmunity are present in large numbers in the injured brain. These data indicate that studies investigating the neuro-immune response after global cerebral ischemia should consider the role of infiltrating T cells in orchestrating the acute and sustained immune response.
机译：尽管炎症机制已与整体脑缺血后神经元损伤有关，但仍未充分研究周围浸润性免疫细胞的存在。我们对由心脏骤停和心肺复苏（CA / CPR）引起的整体性脑缺血后不同时间点从小鼠脑中获得的单细胞悬液进行了流式细胞术，以表征淋巴细胞流入受伤的脑部的特征。我们观察到，CA / CPR在复苏的3小时内引起大量淋巴细胞流入，并在我们实验的3天时间内得以维持。使用细胞染色流式细胞仪，我们观察到大多数浸润淋巴细胞是CD4 + T细胞。细胞内染色显示大部分表达TNFα或INFγ的促炎性T细胞。重要的是，在TCRα基因敲除小鼠中功能性T细胞的缺乏减少了CA / CPR后的神经元损伤，这提示促炎性T细胞参与了缺血性神经元损伤的进展。最后，我们做出了惊人的观察，在受伤的大脑中大量存在与自身免疫密切相关的促炎性T细胞的新型CD4 + CD40 +（Th40）群体。这些数据表明，研究全脑缺血后神经免疫应答的研究应考虑浸润性T细胞在协调急性和持续免疫应答中的作用。

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