Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1 A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro

QUNQUN GUO; DAOSEN GUO; BOGUANG ZHAO; JIE XU; RONGGUI LI

首页> 外文期刊>Journal of Nematology, with Annual of Applied Nematology >Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1 A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro

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Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1 A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro

机译：松材线虫携带的荧光假单胞菌GcM5-1 A的两个环二肽及其对日本黑松悬浮细胞和幼苗的毒性

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Pseudomonas fluorescens GcM5-lA, isolated from the pine wood nematode (PWN), Bursaphelenchus xylophilus, was cultured in Luria Broth medium (LB). The clarified culture was extracted with ethyl acetate, and two dipeptides were purified from the extract. The chemical structures of 1 and 2 were identified as cyclo(-Pro-Val-)and cyclo (-Pro-Tyr-), respectively, by MS, ~1H NMR, ~(13)C NMR, ~1H-~1H COSY, ~1H -~(13)C COSY spectra. Bioassay results showed that the two compounds were toxic to both suspensioncells and seedlings of Pinus thunbergii, which may offer some clues to research the mechanism of pine wilt disease caused by PWN.

机译：从松木线虫（PWN），松材线虫（Bursaphelenchus xylophilus）中分离出的荧光假单胞菌GcM5-1A在Luria Broth培养基（LB）中培养。用乙酸乙酯萃取澄清的培养物，并从萃取物中纯化出两个二肽。通过MS，〜1H NMR，〜（13）C NMR，〜1H-〜1H COZY分别将1和2的化学结构鉴定为环（-Pro-Val-）和环（-Pro-Tyr-）。，〜1H-〜（13）C COYY光谱。生物测定结果表明，这两种化合物对黑松的悬浮细胞和幼苗均具有毒性，这可能为研究PWN引起的松树枯萎病的机理提供一些线索。

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来源
《Journal of Nematology, with Annual of Applied Nematology》 |2007年第3期|共5页
作者
QUNQUN GUO; DAOSEN GUO; BOGUANG ZHAO; JIE XU; RONGGUI LI;
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正文语种 eng
中图分类动物分类学（系统动物学）;
关键词
Bursaphelenchus xylophilus; cyclo (-Pro-Tyr-); cyclo (-Pro-Val-); interaction; Pinus thunbergii; Pseudomonas fluorescens GcM5-1A; toxicity;

机译：嗜木假单胞菌;环（-Pro-Tyr-）;环（-Pro-Val-）;相互作用;雷管;假单胞菌GcM5-1A;毒性;

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1. Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1 A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro [J] . QUNQUN GUO, DAOSEN GUO, BOGUANG ZHAO, Journal of Nematology, with Annual of Applied Nematology . 2007,第3期

机译：松材线虫携带的荧光假单胞菌GcM5-1 A的两个环二肽及其对日本黑松悬浮细胞和幼苗的毒性
2. Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1 A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro [J] . QUNQUN GUO, DAOSEN GUO, BOGUANG ZHAO, Journal of Nematology, with Annual of Applied Nematology . 2007,第3期

机译：松材线虫携带的荧光假单胞菌GcM5-1 A的两个环二肽及其对日本黑松悬浮细胞和幼苗的毒性
3. Purification of flagellin of Pseudomonas fluorescensGcM5-lA carried by the pine wood nematode, Bursaphelenchus xylophilus, and its in vitro toxicity to a suspension of cells of Pinus thunbergii [J] . Daosen Guo, Boguang Zhao, Ronggui Li, Russian Journal of Nematology . 2008,第2期

机译：松材线虫Bursaphelenchus xylophilus携带的荧光假单胞菌鞭毛蛋白的纯化及其对黑松细胞悬浮液的体外毒性
4. In vitro culture of Japanese black pine (Pinus thunbergii) [C] . Katsuaki Ishii, Emilio Maruyama International Symposium on Transplant Production in Closed System for Solving the Global Issues on Environmental Conservation, Food, Resources, and Energy . 2000

机译：日本黑松（Pinus Thunbergii）的体外培养物
5. Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro [O] . Qunqun Guo, Daosen Guo, Boguang Zhao, 2007

机译：松材线虫携带的荧光假单胞菌GcM5-1A的两个环二肽及其对日本黑松悬浮细胞和幼苗的毒性
6. Migration and Population Growth of the Pine Wood Nematode (Bursaphelenchus xylophilus) Related to the Symptom Development in the Seedlings of Japanese Black Pine (Pinus thunbergii) Families Selected as Resistant to Pine Wilt. [O] . K. Kuroda, M. Ohira, M. Okamura, 2007

机译：迁移和人口生长的松木线虫（Bursaphelenchus Xylophilus）与日本黑松（Pinus Thunbergii）植物幼苗的症状发展有关，被选为抗松树枯萎病。

Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1 A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro

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