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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Evaluation of asymmetric immunoliposomal nanoparticles for cellular uptake
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Evaluation of asymmetric immunoliposomal nanoparticles for cellular uptake

机译：评估不对称免疫脂质体纳米颗粒的细胞摄取

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Effective and targeted in vivo delivery of polynucleotide therapeutics is the key for the treatment of many diseases. Asymmetric immunoliposomes can be used as vehicles to deliver polynucleotides effectively because the two leaflets of the bilayer can have different compositions, which enhance the delivery capacity. The formation and in vitro cellular uptake of asymmetric immunoliposomes containing polynucleotide cargoes were studied here. Maleimide-functionalised DSPE-PEG (2000) were incorporated into the outer leaflet to produce asymmetric liposomes capable of covalently attaching antibodies. Thiolated antibodies from both human and rabbit origin were conjugated to produce asymmetric pendant-type immunoliposomes that retain their specificity towards detection antibodies through the formation process. Human IgG-conjugated asymmetric immunoliposomes were readily internalised (>20 per cell) by macrophage, HEPG2, and CV-1 monkey kidney cells. The cells internalised the liposomal nanoparticles by the endocytic pathway. The immunoliposome-encapsulated endosomes were intact for at least 5 days and sequestered the plasmid from expression by the cell.
机译：有效和靶向体内递送多核苷酸治疗剂是治疗许多疾病的关键。不对称的免疫脂质体可以用作有效递送多核苷酸的媒介物，因为双层的两个小叶可以具有不同的组成，从而增强了递送能力。这里研究了含有多核苷酸货物的不对称免疫脂质体的形成和体外细胞摄取。将马来酰亚胺官能化的DSPE-PEG（2000）掺入外部小叶以产生能够共价结合抗体的不对称脂质体。将人和兔来源的硫酰化抗体偶联以产生不对称的悬垂型免疫脂质体，该脂质体在形成过程中仍对检测抗体具有特异性。巨噬细胞，HEPG2和CV-1猴肾细胞很容易将人IgG偶联的不对称免疫脂质体内在化（每个细胞> 20）。细胞通过内吞途径内化脂质体纳米颗粒。完整包裹免疫脂质体的内体至少维持5天，并从细胞中隔离质粒。

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