...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >An understanding of potential and limitations of alginate/PLL microcapsules as a cell retention system for perfusion cultures
【24h】

An understanding of potential and limitations of alginate/PLL microcapsules as a cell retention system for perfusion cultures

机译：了解藻酸盐/ PLL微胶囊作为灌注培养细胞保留系统的潜力和局限性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microcapsules for high cell density culture of mammalian cells have found an increasing interest, however, the poor stability of the microcapsules and the lack of characterisation methods led to few quantitative results. Alginate-poly-L-lysine (PLL) microcapsules have been studied in detail in order to form a basis for comparison of capsules made from different polymers. Since the microcapsules can be easily retained in the bioreactor without the need for a cell separation device, high cell densities were achieved with a maximum of 4x10(7) cell/ml(microcapsules), corresponding to a colonisation of 5% of the internal capsule volume. Measurement of microcapsule integrity and mechanical resistance showed that alginate-PLL microcapsules are not suitable for perfusion cultures since they are very sensitive to media composition, mainly the presence of non-gelling ions that have a higher affinity for alginate than PLL and Ca2+, leading to the leakage of PLL and Ca2+, and to microcapsule rupture.
机译：对于用于哺乳动物细胞的高细胞密度培养的微囊，人们越来越感兴趣，但是，微囊的稳定性差和缺乏表征方法导致很少的定量结果。对藻酸盐-聚-L-赖氨酸（PLL）微胶囊进行了详细研究，以便为比较由不同聚合物制成的胶囊提供基础。由于微胶囊可以轻松地保留在生物反应器中，而无需细胞分离装置，因此最高可实现4x10（7）细胞/ ml（微胶囊）的高细胞密度，相当于内囊定殖的5％卷。微胶囊完整性和机械阻力的测量结果表明，藻酸盐-PLL微胶囊不适合灌注培养，因为它们对培养基组成非常敏感，主要是非凝胶离子的存在，其对藻酸盐的亲和力高于PLL和Ca2 +，导致PLL和Ca2 +的泄漏，以及微囊破裂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号