首页> 外文期刊>Journal of Nematology, with Annual of Applied Nematology >Molecular Characterization and Phylogenetic Analysis of Hoplolaimus stephanus
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Molecular Characterization and Phylogenetic Analysis of Hoplolaimus stephanus

机译:蛇麻草的分子表征和系统发育分析

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Three Hoplolaimus stephanus populations were characterized morphologically, both by morphometrics and by SEM observations. These populations were used to develop a rapid and accurate molecular identification method for the species, which is useful because of the high level of morphological similarity between H. stephanus and H. galeatus. Species-specific primers for H. stephanus, amplifying a distinct fragment (260 bp) of the nuclear ribosomal internal transcribed spacerl (ITS1), can be used in multiplex PCR along with previously developed primers for other common Hoplolaimus species. We also infer phylogenetic relationships among H. stephanus, the closely-related H. galeatus, and several other Hoplolaimus species, using sequences of the actin gene,ITS1 and LSUD.
机译:通过形态计量学和SEM观察,对三个霍普莱穆斯Stephanus种群进行了形态学表征。这些种群被用来为该物种开发一种快速,准确的分子鉴定方法,这是有用的,因为Stephanus和galeatus之间的形态相似性很高。用于Stephanus的物种特异性引物,可扩增核糖体内部转录的spacer(ITS1)的独特片段(260 bp),可与先前开发的其他常见Hoplolaimus引物一起用于多重PCR。我们还使用肌动蛋白基因,ITS1和LSUD的序列,推断出斯蒂芬螺旋体,密切相关的半乳糖类螺旋体和其他几种Hoplolaimus物种之间的系统发育关系。

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