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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Japanese encephalitis virus vaccine formulations using PLA lamellar and PLG microparticles
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Japanese encephalitis virus vaccine formulations using PLA lamellar and PLG microparticles

机译：使用PLA片层和PLG微粒的日本脑炎病毒疫苗制剂

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摘要

Japanese encephalitics virus (JEV)-loaded poly(lactide) (PLA) lamellar and poly(DL-lactide-co-glycolide) (PLG) microparticles were successfully prepared with low molecular weight PLA by the precipitate method and with 6% w/v PLG in the organic phase, 10% w/v PVP and 5% w/v NaCl in the continuous phase, by using a water-in-oil-in-water emulsion/solvent extraction technique, respectively. JEV was entrapped in the PLG microparticles by a solvent extraction technique with trapping efficiencies up to 98%, loading level 5.5% w/w, and mean particle size 3.8 mum. The distribution (%) of JEV on the PLG microparticles surface, outer layer, and core were 11.2, 41.7 and 46.4%, respectively. The cumulative release of JEV had an upper limit of approx 58% of the JEV load at 24 days. The steady release rate was 1.33 mug JEV/mg microparticles/day of JEV release is approx 0.78% w/w and the loading efficiency (77.8%), JEV content (7.84 mug/mg), and yield (96.3%), respectively. The distribution (%) of JEV on the microparticles surface, outer layer, and core were 82.1, 13.3 and 2.2%, respectively. The live JEV challenge in mice test, in which mice received one dose of 20 mg JEV-loaded PLG microparticles, 20 mg JEV-loaded PLA lamellar in comparison with JEV or PBS solution, was evaluated after IP immunization of BALB/c mice. The study results show that the greatest survival was observed in the group of mice immunized with 20 mg JEV-loaded PLG microparticles and 20 mg JEV-loaded PLA microparticles group (80%). The JEV incorporation, physicochemical characterization data, and the animal results obtained in this study may be relevant in optimizing the vaccine incorporation and delivery properties of these potential vaccine targeting carriers.
机译：通过沉淀法，低分子量PLA和6％w / v成功制备了载有日本脑炎病毒（JEV）的聚丙交酯（PLA）薄片和聚（DL-丙交酯-乙交酯乙交酯）（PLG）微粒通过使用水包油包水型乳液/溶剂萃取技术，有机相中的PLG，连续相中的10％w / v PVP和5％w / v NaCl。通过溶剂萃取技术将JEV截留在PLG微粒中，捕集效率高达98％，负载水平为5.5％w / w，平均粒径为3.8微米。 JEV在PLG微粒表面，外层和核心上的分布（％）分别为11.2％，41.7％和46.4％。 JEV的累积释放在24天时的上限约为JEV负载的58％。稳定释放率为1.33马克杯JEV /毫克微粒/天的JEV释放量约为0.78％w / w，装载效率（77.8％），JEV含量（7.84马克杯/毫克）和产量（96.3％）。 JEV在微粒表面，外层和核上的分布（％）分别为82.1、13.3和2.2％。在小鼠免疫试验中对JEV进行实时测试，其中IP免疫BALB / c小鼠后，对小鼠接受一剂20 mg载有JEV的PLG微粒，与JEV或PBS溶液相比，接受了20 mg载有JEV的PLA薄片。研究结果表明，在用20 mg载有JEV的PLG微粒和20 mg载有JEV的PLA微粒免疫的小鼠组中，观察到最大的存活率（80％）。在这项研究中获得的JEV掺入，理化特性数据和动物结果可能与优化这些潜在疫苗靶向载体的疫苗掺入和递送特性有关。

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