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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Microencapsulation of a recombinant aminopeptidase(PepN)from Lactobacillus rhamnosus S93 in chitosan-coated alginate beads
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Microencapsulation of a recombinant aminopeptidase(PepN)from Lactobacillus rhamnosus S93 in chitosan-coated alginate beads

机译:鼠李糖乳杆菌S93重组氨基肽酶(PepN)在壳聚糖包被的藻酸盐微珠中的微囊化

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摘要

A recombinant aminopeptidase(90 kDa)of Lactobacillus rhamnosus S93 produced by E.coli was encapsulated in alginate or chitosan-treated alginate beads prepared by an extrusion method.This study investigated the effects of alginate,CaCl2,chitosan concentrations,hardening time,pH and alginate/enzyme ratios on the encapsulation efficiency(EE)and the enzyme release(ER).Chitosan in the gelling solution significantly increased the EE from 30.2%(control)to 88.6%(coated).This polycationic polymer retarded the ER from beads during their dissolution in release buffer.An increase in alginate and chitosan concentrations led to greater EE and lesser ER from the beads.The greatest EE was observed in a pH 5.4 solution(chitosan-CaCl2)during bead formation.Increasing the CaCl2 concentration over 0.1 M neither affected the EE nor the ER.Increasing hardening time beyond lOmin led to a decrease in EE and the alginate:enzyme ratio(3:1)was optimal to prevent the ER.
机译:将大肠杆菌生产的鼠李糖乳杆菌S93重组氨肽酶(90 kDa)包埋在藻酸盐或壳聚糖处理的藻酸盐微珠中,研究了藻酸盐,CaCl2,壳聚糖浓度,硬化时间,pH和pH的影响。海藻酸钠/酶比对包封效率(EE)和酶释放(ER)的影响。胶凝溶液中的壳聚糖将EE从30.2%(对比例)显着提高至88.6%(被膜)。它们在释放缓冲液中的溶解。藻酸盐和壳聚糖浓度的增加导致珠粒的EE增大而ER减小。在珠粒形成过程中,pH 5.4溶液(壳聚糖-CaCl2)中观察到最大的EE.CaCl2浓度增加超过0.1M超过10min的淬火时间增加会导致EE降低,而藻酸盐:酶比(3:1)最适合防止ER。

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