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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Hydrophobic microspheres for in situ removal of 2-phenylethanol from yeast fermentation
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Hydrophobic microspheres for in situ removal of 2-phenylethanol from yeast fermentation

机译：疏水微球从酵母发酵中原位去除2-苯基乙醇

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摘要

The commercial production of the fragrance compound 2-phenylethanol (2-PE) from phenylalanine by yeast is limited by the accumulation of the toxic product, and therefore, in situ product removal techniques are required. We describe the use of hydrophobic polymethylmethacrylate (PMMA) microspheres of narrow size distribution of 1.53 ±0.10 urn diameter for continuous removal of 2-PE from the fermentation medium by a mechanism of swelling. In shake flask experiments with conditions simulating 2-PE stress, a 10-fold increase in productivity was measured for systems containing >10% (w/v) microspheres. A 1 L fed-batch fermentation with 8% (w/v) of PMMA microspheres resulted in a total 2-PE concentration of 7.05 g/L, from which 5.40 g/L was incorporated inside the resin, implying 76% encapsulation. This ratio of 0.07 g/g of product per resin is among the highest reported to date. Scanning electron microscopy revealed a concomitant increase in sphere diameter confirming that swelling occurred.
机译：酵母从苯丙氨酸中商业生产芳香化合物2-苯乙醇（2-PE）受有毒产物的积累限制，因此，需要就地去除产物的技术。我们描述了使用疏水的聚甲基丙烯酸甲酯（PMMA）微球，其直径分布狭窄，直径为1.53±0.10微米，用于通过溶胀机制从发酵培养基中连续去除2-PE。在具有模拟2-PE应力的条件的摇瓶实验中，对于包含> 10％（w / v）微球的系统，生产率提高了10倍。用8％（w / v）的PMMA微球进行的1 L分批补料发酵导致2-PE的总浓度为7.05 g / L，从中将5.40 g / L掺入树脂中，意味着76％的包封率。每种树脂0.07 g / g产品的比率是迄今为止报道的最高比率。扫描电子显微镜显示，球体直径随之增加，确认发生了肿胀。

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