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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Encapsulation of poly(D, L-lactide) microparticles with polyelectrolyte multilayers for antigen delivery
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Encapsulation of poly(D, L-lactide) microparticles with polyelectrolyte multilayers for antigen delivery

机译：聚（D，L-丙交酯）微粒与聚电解质多层封装的抗原传递

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摘要

Poly(D, L-lactide) (PLA) microparticles containing the ovalbumin (OVA) model antigen were prepared by the double-emulsion and solvent evaporation method, followed by encapsulation with alternating layers of the polyelectrolytes, consisting of protamine sulfate and dextran sulfate of various molecular weights. The physicochemical properties, including particle size and zeta potentials, were characterised. Treatment of mouse macrophages with surface-modified PLA microparticles stimulated the generation of reactive oxygen species (ROS), such as hydrogen peroxide and superoxide, which was detected by the fluorescent probes, 2 ',7 '-dichlorofluorescein diacetate (DCFH-DA) and hydroethidine (HE). Incubation of murine bone marrow-derived dendritic cells (BMDCs) with the encapsulated PLA microparticles enhanced the presentation of OVA soluble antigens in B3Z cells, an OVA-specific CD8(+) T cell hybridoma. Results obtained in this study demonstrated the potential use of polyelectrolyte-encapsulated biodegradable microparticles for delivery of soluble antigens to the antigen presenting cells and stimulation of effective antigen presentation in the context of class I major histocompatibility complex.
机译：含有卵清蛋白（OVA）模型抗原的聚（D，L-丙交酯）（PLA）微粒是通过双乳液和溶剂蒸发法制备的，然后交替包封由硫酸鱼精蛋白和硫酸葡聚糖组成的聚电解质层各种分子量。表征其物理化学性质，包括粒径和ζ电位。用表面修饰的PLA微粒处理小鼠巨噬细胞刺激了活性氧（ROS）的生成，例如过氧化氢和超氧化物，通过荧光探针2'，7'-dichlorofluorescein diacetate（DCFH-DA）和氢乙啶（HE）。用封装的PLA微粒孵育鼠源性骨髓树突状细胞（BMDC）可增强B3Z细胞（OVA特异性CD8（+）T细胞杂交瘤）中OVA可溶性抗原的表达。在这项研究中获得的结果表明，在I类主要组织相容性复合物的背景下，聚电解质包裹的可生物降解微粒可用于将可溶性抗原递送至抗原呈递细胞并刺激有效抗原呈递。

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