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首页> 外文期刊>Biomaterials >The effect of poly(d,l-lactide-co-glycolide) microparticles with polyelectrolyte self-assembled multilayer surfaces on the cross-presentation of exogenous antigens.
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The effect of poly(d,l-lactide-co-glycolide) microparticles with polyelectrolyte self-assembled multilayer surfaces on the cross-presentation of exogenous antigens.

机译：具有聚电解质自组装多层表面的聚（d，l-丙交酯-共-乙交酯）微粒对外源抗原交叉呈递的影响。

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A surface-engineered particulate delivery system for exogenous antigens was developed in this study. Poly(d,l-lactide-co-glycolide) (PLGA) microparticles containing ovalbumin (OVA) or fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) were fabricated by the double emulsion and solvent evaporation method. Encapsulation of the PLGA microparticles was performed by physisorption of multilayers of oppositely charged polyelectrolytes, including polyethylenimine (PEI) and dextran sulfate. Surface charges of the particles after layer-by-layer (LbL) adsorption were determined by the zeta potential measurements. The uptake of these particles by the J774A.1 murine macrophages was examined by fluorescence microscopy. Generation of reactive oxygen species (ROS) in J774A.1 cells was determined by flow cytometry using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and hydroethidine (HE). Antigen presentation assays were performed in B3Z cells, a hybridoma of OVA-specific CD8(+) T cells. Results obtained in this study demonstrated an effective ingestion of the PLGA microparticles and enhanced production of ROS in J774A.1 murine macrophages. Treatment of murine bone marrow-derived dendritic cells (BMDCs) with polyelectrolyte-encapsulated PLGA microparticles resulted in an in vitro activation of B3Z cells, demonstrating the feasibility of induction of adaptive immunity for class I major histocompatibility complex (MHC) by surface engineering of microparticulate vaccine delivery.
机译：在这项研究中开发了一种表面工程化的外源抗原颗粒输送系统。通过双重乳液和溶剂蒸发法制备了包含卵清蛋白（OVA）或荧光素异硫氰酸酯共轭的牛血清白蛋白（FITC-BSA）的聚（d，l-丙交酯-乙交酯）（PLGA）微粒。 PLGA微粒的封装是通过对带有相反电荷的聚电解质（包括聚乙烯亚胺（PEI）和硫酸葡聚糖）进行多层物理吸附来完成的。通过zeta电位测量确定吸附（LbL）后颗粒的表面电荷。通过荧光显微镜检查J774A.1鼠巨噬细胞对这些颗粒的摄取。使用2'，7'-二氯二氢荧光素二乙酸酯（DCFH-DA）和氢乙啶（HE）通过流式细胞术确定J774A.1细胞中活性氧（ROS）的产生。抗原呈递分析是在B3Z细胞（OVA特异性CD8（+）T细胞的杂交瘤）中进行的。这项研究获得的结果表明，J774A.1鼠巨噬细胞可有效摄入PLGA微粒并增强ROS的产生。用聚电解质包裹的PLGA微粒处理鼠源性树突状细胞（BMDC）导致B3Z细胞的体外活化，证明了通过微粒表面工程诱导I类主要组织相容性复合物（MHC）适应性免疫的可行性。疫苗运送。

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