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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Development of alpha-lipoic acid encapsulated chitosan monodispersed particles using an electrospray system: synthesis, characterisations and anti-inflammatory evaluations
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Development of alpha-lipoic acid encapsulated chitosan monodispersed particles using an electrospray system: synthesis, characterisations and anti-inflammatory evaluations

机译：使用电喷雾系统开发α-硫辛酸包裹的壳聚糖单分散颗粒：合成，表征和抗炎评估

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摘要

This study demonstrates the feasibility of using a single-capillary electrospray (ES) system to generate novel alpha-lipoic acid encapsulated poly(ethylene oxide)-chitosan (ALA/PEO/CS) particles with a monodispersed diameter. Scanning electron microscopic images (SEM) and dynamic light scattering (DLS) results indicate that the ES system can generate either a dry powder or a homogeneous water-based suspension of ALA/PEO/CS particles. The SEM images revealed that the ALA/PEO/CS particles have a spherical shape with a diameter of approximately 707 +/- 66.68 nm, and DLS showed that the ALA/PEO/CS particles suspended in deionised water have a diameter of 734.5 nm. In addition, zeta potential studies were performed using a zetasizer instrument and showed positively electric surface potential of 57.7 +/- 0.5 mV, which was attributed to chitosan. Based on the DLS and zeta potential studies, we concluded that the excellent dispersity and stability of the ALA/PEO/CS suspension is attributed to the reduction in particle size and electrostatic repulsion between these tiny particles. Finally, we used lipopolysaccharide (LPS)-induced nitrite formation in Raw 264.7 macrophages as a model for in vitro anti-inflammation evaluation. We find that the anti-inflammatory ability of the ALA/PEO/CS particles is superior to that of free ALA solution in macrophage cells, which is attributed to the more efficiently intracellular delivery. The confocal image results prove that the uptake of ALA/PEO/CS particles by the LPS-treated Raw 264.7 macrophages is possibly initiated by the interaction with cell-surface molecules through electrostatic interactions, followed by endocytosis of the attached particles.
机译：这项研究证明了使用单毛细管电喷雾（ES）系统生成具有单分散直径的新型α-硫辛酸包封的聚环氧乙烷-壳聚糖（ALA / PEO / CS）颗粒的可行性。扫描电子显微镜图像（SEM）和动态光散射（DLS）结果表明，ES系统可生成ALA / PEO / CS颗粒的干粉或均质水基悬浮液。 SEM图像显示ALA / PEO / CS颗粒具有直径约为707 +/- 66.68nm的球形，并且DLS显示悬浮在去离子水中的ALA / PEO / CS颗粒具有734.5nm的直径。此外，使用zetasizer仪器进行了ζ电位研究，结果表明，归因于壳聚糖的表面正电位为57.7 +/- 0.5 mV。基于DLS和zeta电位研究，我们得出结论，ALA / PEO / CS悬浮液的优异分散性和稳定性归因于这些微小颗粒之间的粒径减小和静电排斥。最后，我们将脂多糖（LPS）诱导的Raw 264.7巨噬细胞中亚硝酸盐的形成用作体外抗炎评估的模型。我们发现，巨噬细胞中ALA / PEO / CS颗粒的抗炎能力优于游离ALA溶液，这归因于更有效的细胞内传递。共聚焦图像结果证明，LPS处理的Raw 264.7巨噬细胞对ALA / PEO / CS颗粒的吸收可能是通过与细胞表面分子的静电相互作用，然后是附着颗粒的内吞作用而引发的。

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