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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >A novel approach for antibody nanocarriers development through hydrophobic ion-pairing complexation
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A novel approach for antibody nanocarriers development through hydrophobic ion-pairing complexation

机译：通过疏水离子配对络合开发抗体纳米载体的新方法

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摘要

IgG-Fab fragment, a model antibody protein was hydrophobically modified by a novel approach of ion-pairing complexation. Three different sulphated ion-pairing agents were utilised including sodium dodecyl sulphate, taurocholic acid and dextran sulphate (DS). The formations of hydrophobic ion-pairing (HIP) complexes were dependant on pH and molar ratio of ion-pairing agent to Fab. Aqueous solubilities of HIP complexes were very low compared to Fab alone. In particular, when dextran sulphate was added as ion-pairing agent, formed Fab: DS HIP complexes were least soluble in water. Further, nanoparticles (NPs) loaded with drug and Fab: DS HIP complex were prepared and characterised with respect to encapsulation efficiency and size. We observed significant improvement in encapsulation efficiency for Fab: DS HIP complex-loaded nanoparticles. This study demonstrates a novel approach of formulating antibody-loaded nanoparticles which can also be employed for delivery of large antibodies.
机译：IgG-Fab片段，一种模型抗体蛋白，通过离子对络合的新方法进行了疏水修饰。使用了三种不同的硫酸化离子对试剂，包括十二烷基硫酸钠，牛磺胆酸和葡聚糖硫酸盐（DS）。疏水离子对（HIP）配合物的形成取决于pH和离子对剂与Fab的摩尔比。与单独的Fab相比，HIP复合物的水溶性很低。特别地，当添加硫酸葡聚糖作为离子配对剂时，形成的Fab：DS HIP复合物在水中的溶解度最低。此外，制备了载有药物和Fab：DS HIP复合物的纳米颗粒（NP），并就包封效率和尺寸进行了表征。我们观察到Fab：DS HIP复合物负载纳米颗粒的封装效率有了显着提高。这项研究证明了配制载有抗体的纳米颗粒的新方法，该方法也可用于递送大抗体。

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