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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Synthesis and in vitro study of cisplatin-loaded Fe3O4 nanoparticles modified with PLGA-PEG(6000) copolymers in treatment of lung cancer
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Synthesis and in vitro study of cisplatin-loaded Fe3O4 nanoparticles modified with PLGA-PEG(6000) copolymers in treatment of lung cancer

机译：PLGA-PEG（6000）共聚物修饰的顺铂负载Fe3O4纳米粒子的合成及体外研究

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In the field of cancer therapy, magnetic nanoparticles modified with biocompatible copolymers are promising vehicles for the delivery of hydrophobic drugs such as Cisplatin. The major aim of this effort was to evaluate whether Cisplatin-Encapsulated magnetic nanoparticles improved the anti-tumour effect of free Cisplatin in lung cancer cells. The PLGA-PEG triblock copolymer was synthesised by ring-opening polymerisation of D,L-lactide and glycolide with polyethylene glycol (PEG(6000)) as an initiator. The bulk properties of these copolymers were characterised using Fourier transform infrared spectroscopy. Cisplatin-loaded nanoparticles (NPs) were prepared by double emulsion solvent evaporation technique and were characterised for size, drug entrapment efficiency (%), drug content (% w/w), and surface morphology. In vitro release profile of cisplatin-loaded NP formulations was determined. Cytotoxic assays were evaluated in lung carcinoma (A549)-treated cells by the MTT assay technique. In addition, the particles were characterised by X-ray powder diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometry. The anti-proliferative effect of Cisplatin appeared much earlier when the drug was encapsulated in magnetic nanoparticles than when it was free. Cisplatin-Encapsulated magnetic nanoparticles significantly enhanced the decrease in IC50 rate. The in vitro cytotoxicity test showed that the Fe3O4-PLGA-PEG(6000) magnetic nanoparticles had no cytotoxicity and were biocompatible. The chemotherapeutic effect of free Cisplatin on lung cancer cells is improved by its encapsulation in modified magnetic nanoparticles. This approach has the prospective to overcome some major limitations of conventional chemotherapy and may be a promising strategy for future applications in lung cancer therapy.
机译：在癌症治疗领域，用生物相容性共聚物改性的磁性纳米粒子是用于输送疏水性药物（如顺铂）的有前途的载体。这项工作的主要目的是评估用顺铂包裹的磁性纳米粒子是否能改善游离顺铂在肺癌细胞中的抗肿瘤作用。 PLGA-PEG三嵌段共聚物是通过D，L-丙交酯和乙交酯与聚乙二醇（PEG（6000））作为引发剂的开环聚合反应合成的。使用傅立叶变换红外光谱法表征这些共聚物的本体性能。通过双乳液溶剂蒸发技术制备了负载顺铂的纳米颗粒（NPs），并对其尺寸，药物截留效率（％），药物含量（％w / w）和表面形态进行了表征。确定了载有顺铂的NP制剂的体外释放曲线。通过MTT测定技术评价了在肺癌（A549）处理的细胞中的细胞毒性测定。另外，通过X射线粉末衍射，扫描电子显微镜，傅立叶变换红外光谱和振动样品磁力测定法对颗粒进行表征。当药物被包裹在磁性纳米颗粒中时，顺铂的抗增殖作用要比游离时更早。顺铂封装的磁性纳米粒子显着增强了IC50速率的降低。体外细胞毒性试验表明，Fe3O4-PLGA-PEG（6000）磁性纳米粒子无细胞毒性，具有生物相容性。游离顺铂封装在修饰的磁性纳米粒子中可改善其对肺癌细胞的化学治疗作用。这种方法有望克服常规化学疗法的一些主要局限性，并且可能是未来在肺癌治疗中应用的有希望的策略。

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