• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Encapsulation of plasmid DNA in PLGA-stearylamine microspheres: a comparison of solvent evaporation and spray-drying methods
【24h】

Encapsulation of plasmid DNA in PLGA-stearylamine microspheres: a comparison of solvent evaporation and spray-drying methods

机译：PLGA-硬脂胺微球中质粒DNA的封装：溶剂蒸发和喷雾干燥方法的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Stearylamine, a positively charged hydrophobic molecule, was tested as a formulation agent for the encapsulation of a model plasmid in PLGA microspheres. The primary objective was to compare the spra-drying and double emulsion solvent evaporation methods and evaluate their suitability for fabricating PLGA-stearylamine plamid-entrapped microspheres. A luciferase reporter gene plasmid (pGL3-Con) was formulated into mirosp9heres using a 64 kDa PLGA 50:50 polymer blended with stearylamine (SA) at a range of concentrations up to 15% m/m, by the solvent evaporation and spray-drying methods. The microspheres were characterized regarding their size distributions, zeta potentials and morphology by laser diffraction, electrophoretic mobility and scanning electron microscopy (SEM), respectively. Formulated plasmid extracts were assessed for physical damage by agarose gel electrophoresis, and the in vitro biological activity was determined by tranfectio of a human embryo kidney epithelial (293) cell line. Size distribution analysis showed that SA reduced the median diameters of spray-dried particles from 8.32 to 3.64 microns, with a corresponding reduction in the spread of the distribution, but solvent evaporation microspheres showed an increased median diameter on addition of SA. Concentrations of SA above 10% m/m resulted in disruption of the smooth morphology of the solvent evaporation particles. There was a SA concentration-dependent tendency in the increase of surface positive charge and resistance to serum nuclease assault and in vitro expression of luciferase protein. These results show that SA and possibly other charged hydrophobic molecules may be useful agents in the formulation of particulate DNA vaccines by both methods.
机译：测试了硬脂胺（一种带正电荷的疏水分子）作为一种制剂试剂，用于将模型质粒封装在PLGA微球中。主要目的是比较喷雾干燥法和双乳液溶剂蒸发法，并评估其在制备包裹有PLGA-硬脂基胺糊精的微球中的适用性。通过将64kDa PLGA 50:50聚合物与硬脂胺（SA）以高达15％m / m的浓度范围混合，通过溶剂蒸发和喷雾干燥，将荧光素酶报告基因质粒（pGL3-Con）配制成mirosp9方法。通过激光衍射，电泳迁移率和扫描电子显微镜（SEM）分别对微球的尺寸分布，ζ电势和形态进行了表征。通过琼脂糖凝胶电泳评估配制的质粒提取物的物理损伤，并通过人胚肾上皮（293）细胞系的转染确定体外生物活性。尺寸分布分析表明，SA将喷雾干燥颗粒的中值直径从8.32微米减小到3.64微米，分布的分布也相应减小，但是溶剂蒸发微球在添加SA时显示出中值直径的增加。 SA的浓度高于10％m / m导致破坏溶剂蒸发颗粒的光滑形态。在表面正电荷的增加和对血清核酸酶攻击以及荧光素酶蛋白的体外表达的抗性中，存在SA浓度依赖性的趋势。这些结果表明，通过两种方法，SA和可能带有其他电荷的疏水性分子在微粒DNA疫苗制剂中可能都是有用的试剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号