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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Cationic stearylamine-containing biodegradable microparticles for DNA delivery
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Cationic stearylamine-containing biodegradable microparticles for DNA delivery

机译：用于DNA传递的含阳离子硬脂胺的可生物降解微粒

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摘要

Technical aspects for preparing a new type of cationic stearylamine (SA)-con-taining microparticle as a potential drug delivery system for negatively charged therapeutics were investigated.Cationic biodegradable microparticles based on poly(lactide) and poly(lactide-co-glycolide) were prepared upon incorporation of SA either by solvent evaporation or by spray-drying.Water-insoluble SA offers the advantage over other water-soluble cationic compounds that it can be dissolved directly in the organic solution together with the polymers.This facilitated the subsequent preparation of the microparticle formulations.Particle size was controlled by the respective process parameters,resulting in either large polymer aggregates within the range 50-100 mum or small spherical microparticles writhin the range 1-10 mum.The incorporation of SA into the formulations also improved particle characteristics in terms of re-dispersibility,reduced sticking,and particle size uniformity.Both circular plasmid DNA (5 kbp) and linear salmon DNA (0.5 kbp) were efficiently adsorbed to the cationic SA microparticle surfaces.Preliminary tests on the release of DNA from spray-dried SA microparticles showed an immediate burst release,which was followed by a delayed second release phase for more than 4 weeks.The cationic SA microparticles might provide a potential drug-delivery system to improve the efficacy for protein and DNA-type therapeutics.
机译：研究了制备新型含阳离子硬脂胺（SA）的微粒作为带负电荷治疗剂的潜在药物递送系统的技术方面。基于聚丙交酯和聚丙交酯-乙交酯的阳离子可生物降解微粒为水不溶性SA与其他水溶性阳离子化合物相比具有优势，可以与溶剂一起直接溶于有机溶液中，这有利于后续的制备颗粒尺寸由各自的工艺参数控制，结果是在50-100微米范围内的大聚合物聚集体或在1-10微米范围内的小球形微粒。将SA掺入配方中还改善了颗粒特性在可再分散性，减少粘附和粒径均匀性方面。 NA（5 kbp）和线性鲑鱼DNA（0.5 kbp）被有效地吸附到阳离子SA微粒表面上。对喷雾干燥SA微粒中DNA释放的初步测试显示立即爆发释放，然后延迟第二次释放阳离子SA微粒可能会提供一个潜在的药物递送系统，以提高蛋白质和DNA型治疗剂的疗效。

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