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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >In-vitro analysis of APA microcapsules for oral delivery of live bacterial cells
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In-vitro analysis of APA microcapsules for oral delivery of live bacterial cells

机译：APA微胶囊的体外分析，用于口服活细菌细胞

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摘要

Oral administration of microcapsules containing live bacterial cells has potential as an alternative therapy for several diseases.This article evaluates the suitability of the alginate-poly-L-lysine-alginate (APA) microcapsules for oral delivery of live bacterial cells,in-vitro,using a dynamic simulated human gastro-intestinal (GI) model.Results showed that the APA microcapsules were morphologically stable in the simulated stomach conditions,but did not retain their structural integrity after a 3-day exposure in simulated human GI media.The microbial populations of the tested bacterial cells and the activities of the tested enzymes in the simulated human GI suspension were not substantially altered by the presence of the APA microcapsules,suggesting that there were no significant adverse effects of oral administration of the APA microcapsules on the flora of the human gastrointestinal tract.When the APA microcapsules containing Lactobacillus plantarum 80 (LP80) were challenged in the simulated gastric medium (pH=2.0),80.0% of the encapsulated cells remained viable after a 5-min incubation;however,the viability decreased considerably (8.3%) after 15 min and dropped to 2.6% after 30 min and lower than 0.2% after 60 min,indicating the limitations of the currently obtainable APA membrane for oral delivery of live bacteria.Further in-vivo studies are required before conclusions can be made concerning the inadequacy of APA microcapsules for oral delivery of live bacterial cells.
机译：口服含活细菌细胞的微囊有可能作为多种疾病的替代疗法。本文评估了藻酸盐-聚-L-赖氨酸-藻酸盐（APA）微囊在体外口服活细菌细胞的适用性，结果显示，APA微胶囊在模拟胃部条件下形态稳定，但在模拟人类GI介质中暴露3天后仍未保持其结构完整性。 APA微胶囊的存在不会显着改变模拟人GI悬浮液中被测细菌细胞的数量和被测酶的活性，这表明口服APA微胶囊对细菌的菌群没有明显的不利影响。当人体中含有植物乳杆菌80（LP80）的APA微胶囊受到攻击时模拟胃液（pH = 2.0），孵育5分钟后仍有80.0％的被包封细胞保持活力;但是15分钟后活力显着降低（8.3％），30分钟后降至2.6％，低于0.2％ 60分钟后，表明目前可获得的APA膜对活细菌口服给药的局限性。需要进行进一步的体内研究，才能得出有关APA微胶囊对活细菌细胞口服给药不足的结论。

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