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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Development of a novel casein-protamine based microparticles for early feeding of fish larvae:In vitro evaluation
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Development of a novel casein-protamine based microparticles for early feeding of fish larvae:In vitro evaluation

机译：一种新型酪蛋白鱼精蛋白微粒的开发，用于鱼幼体的早期喂养：体外评价

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Objective:The objective of this study was to develop novel type of protein walled microparticles suitable for using in early feeding of fish larvae.Methods:The microparticles were made of casein and protamine through complex coacervation and did not require further cross-linking or use of environmentally problematic reagents.The methodology was oriented to generate microparticles with an appropriate size range for easy recognition and ingestion by fish larvae(50-200 mu m),adequate floating properties in saline,sufficient stability in terms of protein leakage and appropriate digestibility by the gut enzymes of fish larvae.Results:Desired particle size and stability against protein leakages over 8 h were successfully achieved by optimizing the coacervation process conditions.The floating properties under static conditions were considered appropriate as a main particle fraction remained in suspension during at least 10 min.Very importantly,an enzyme extract from larval gut readily digested the particles.The digestibility of the casein-protamine particles was similar to that measured for Anemia nauplii and for two previously developed casein-based microparticles produced by interfacial polymerization and ionic gelation;the latter microparticle type had previously achieved good results of digestibility in early feeding of marine fish larvae.Conclusion:The in vitro evaluation of the newly developed casein-protamine microparticles revealed promising characteristics as artificial larval feed.Thus,these particles merit further development with respect to entrapping nutrients and testing them in larval cultures for their nutritional value.
机译：目的：本研究的目的是开发适用于鱼类幼体早期饲喂的新型蛋白壁微粒。方法：微粒通过酪蛋白和鱼精蛋白的复合凝聚制成，不需要进一步的交联或使用该方法旨在产生尺寸范围合适的微粒，以易于鱼幼虫（50-200μm）识别和摄取，在盐水中具有足够的漂浮性，在蛋白质泄漏方面具有足够的稳定性，并且可以通过适当的消化消化。结果：通过优化凝聚过程条件成功地实现了8小时以上的所需粒径和对蛋白质泄漏的稳定性。在静态条件下的漂浮性被认为是适当的，因为至少10秒钟内主要颗粒部分保留在悬浮液中最重要的是，幼虫肠道中的酶提取物易于消化酪蛋白鱼精蛋白颗粒的消化率与贫血无节幼体和通过界面聚合和离子凝胶化制备的两种先前开发的酪蛋白基微粒的消化率相似;后者的微粒类型先前在早期已取得了良好的消化率结果结论：对新开发的酪蛋白鱼精蛋白微粒的体外评估显示了其作为人工幼体饲料的良好前景。因此，这些微粒在捕获营养物和在幼体培养物中测试其营养价值方面值得进一步发展。 。

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