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首页> 外文期刊>Journal of Micromechanics and Microengineering >POROUS SILICON AS THE CARRIER MATRIX IN MICROSTRUCTURED ENZYME REACTORS YIELDING HIGH ENZYME ACTIVITIES
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POROUS SILICON AS THE CARRIER MATRIX IN MICROSTRUCTURED ENZYME REACTORS YIELDING HIGH ENZYME ACTIVITIES

机译：多孔硅作为微结构化酶反应器的载体矩阵，可产生高酶活

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Miniaturization and silicon integration of micro enzyme reactors for applications in micro total analysis systems (mu TASs) require new methods to achieve structures with a large surface area onto which the enzyme can be coupled. This paper describes a method to accomplish a highly efficient silicon microstructured enzyme reactor utilizing porous silicon as the carrier matrix. The enzyme activity of microreactors with a porous layer was recorded and compared with a microreactor without the porous layer. The microreactors were fabricated as flow-through cells comprising 32 channels, 50 mu m wide, spaced 50 mu m apart and 250 mu m deep micromachined in [110] oriented silicon, p type (20-70 Omega cm), by anisotropic wet etching. The overall dimension of the microreactors was 13.1 x 3.15 mm. To make the porous silicon layer, the reactor structures were anodized in a solution of hydrofluoric acid and ethanol. In order to evaluate the surface enlarging effect of different pore morphologies, the anodization was performed at three different current densities, 10, 50 and 100 mA cm(-2). Glucose oxidase was immobilized onto the three porous microreactors and a non-porous reference reactor. The enzyme activity of the reactors was monitored following a colorimetric assay. To evaluate the glucose monitoring capabilities, the reactor anodized at 50 mA cm(-2) was connected to an FIA system for glucose monitoring. The system displayed a linear response of glucose up to 15 mM using an injection volume of 0.5 mu l. The result from the studies of glucose turn-over rate clearly demonstrates the potential of porous silicon as a surface enlarging matrix for micro enzyme reactors. An increase in enzyme activity by a factor of 100, compared to the non-porous reference, was achieved for the reactor anodized at 50 mA cm(-2). [References: 16]
机译：用于微型全分析系统（mu TAS）的微型酶反应器的小型化和硅集成需要新的方法来实现具有可与酶偶联的大表面积的结构。本文介绍了一种利用多孔硅作为载体基质完成高效硅微结构化酶反应器的方法。记录具有多孔层的微反应器的酶活性，并将其与不具有多孔层的微反应器进行比较。微反应器通过各向异性湿法刻蚀加工成流通池，包括32个通道，50μm宽，相距50μm和250μm深，在各向异性[110]取向的p型硅（20-70 Omega cm）中进行了微加工。 。微型反应器的整体尺寸为13.1 x 3.15毫米。为了制造多孔硅层，将反应器结构在氢氟酸和乙醇的溶液中进行阳极氧化。为了评估不同孔隙形态的表面放大效果，在三种不同的电流密度（10、50和100 mA cm（-2））下进行了阳极氧化处理。葡萄糖氧化酶固定在三个多孔微反应器和一个无孔参考反应器上。比色测定法监测反应器的酶活性。为了评估葡萄糖监测能力，将在50 mA cm（-2）阳极氧化的反应器连接到FIA系统进行葡萄糖监测。该系统使用0.5μl的进样量显示出高达15 mM的葡萄糖线性响应。葡萄糖周转率研究的结果清楚地表明了多孔硅作为微酶反应器表面放大基质的潜力。与无孔参考相比，在50 mA cm（-2）阳极氧化的反应器中，酶活性提高了100倍。 [参考：16]

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