In the present study, a novel droplet-merging technique is proposed, which enables two nanoliter or picoliter droplets to merge in a straight microchannel with high precision. Two dispersed phase fluids are supplied respectively from two side channels in a certain range of flow rates to generate droplets of different sizes or different viscosities in a regularly alternating mode at a cross-channel intersection prior to the straight microchannel, in such a manner that the droplets are spontaneously synchronized by themselves due to the competition of the two interfaces formed at the cross-channel intersection. Then they are autogeneously merged in the downstream straight microchannel with or without a sudden expansion of the cross section, due to their velocity difference which is induced as they are transported aboard the continuous phase fluid. This droplet-merging method has no desynchronization or secondary-merging problems. Thus, it can be applied efficiently to mixing or encapsulating one target sample with another material for the purposes of nanoparticle synthesis, hydrogel-bead production, cell transplantation and so forth.
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