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首页> 外文期刊>Journal of Neurophysiology >Isolation of the kernel for respiratory rhythm generation in a novel preparation: the pre-Botzinger complex 'island'.
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Isolation of the kernel for respiratory rhythm generation in a novel preparation: the pre-Botzinger complex 'island'.

机译：在新的制剂中分离出用于产生呼吸节律的内核：前波津格复合体“岛”。

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The pre-Botzinger complex (pre-BotC), a bilaterally distributed network of rhythmogenic neurons within the ventrolateral medulla, has been proposed to be the critical locus for respiratory rhythm generation in mammals. To date, thin transverse medullary slice preparations that capture the pre-BotC have served as the optimal experimental model to study the region's inherent cellular and network properties. We have reduced the thin slices to isolated pre-BotC "islands" to further establish whether the pre-BotC has intrinsic rhythmicity and is the kernel for rhythmogenesis in the slice. We recorded neuron population activity locally in the pre-BotC with macroelectrodes and fluorescent imaging of Ca(2+) activities with Calcium Green-1AM dye before and after excising the island. The isolated island remained rhythmically active with a population burst profile similar to the inspiratory burst in the slice. Rhythmic population activity persisted in islands after block of GABA(A)ergic and glycinergic synaptic inhibition. The loci of pre-BotC Ca(2+) activity imaged in thin slices and islands were similar, and imaged pre-BotC neurons exhibited synchronized flashing after blocking synaptic inhibition. Population burst frequency increased monotonically as extracellular potassium concentration was elevated, consistent with mathematical models consisting entirely of an excitatory network of synaptically coupled pacemaker neurons with heterogeneous, voltage-dependent bursting properties. Our results provide further evidence for a rhythmogenic kernel in the pre-BotC in vitro and demonstrate that the islands are ideal preparations for studying the kernel's intrinsic properties.
机译：已经提出，前伯钦格复合体（pre-BotC）是腹侧延髓内有节律性神经元的双边分布网络，是哺乳动物呼吸节律产生的关键场所。迄今为止，捕获pre-BotC的横向髓质薄片已成为研究该地区固有细胞和网络特性的最佳实验模型。我们将薄片缩减为孤立的pre-BotC“岛”，以进一步确定pre-BotC是否具有固有的节律性，并且是切片中节律发生的核心。我们在切除岛屿之前和之后，用大电极在前BotC中记录了局部神经元种群活动，并用钙绿1AM染料对Ca（2+）活动进行了荧光成像。孤岛保持有节奏的活动性，种群爆发特征类似于切片中的吸气爆发。在阻断GABA（A）能和甘氨酸能突触抑制后，岛屿上的节律性种群活动持续存在。在薄片和岛中成像的pre-BotC Ca（2+）活动的基因座是相似的，并且成像的pre-BotC神经元在阻止突触抑制后显示同步闪烁。随着细胞外钾浓度的升高，群体爆发频率单调增加，这与完全由具有异质，电压依赖性爆发特性的突触起搏器神经元的兴奋性网络组成的数学模型一致。我们的结果为pre-BotC体外具有节律性的核提供了进一步的证据，并证明这些岛是研究核的内在特性的理想准备。

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