...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neurophysiology >Mouse taste cells with glialike membrane properties.
【24h】

Mouse taste cells with glialike membrane properties.

机译：具有胶质样膜特性的小鼠味觉细胞。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Taste buds are sensory structures made up by tightly packed, specialized epithelial cells called taste cells. Taste cells are functionally heterogeneous, and a large proportion of them fire action potentials during chemotransduction. In view of the narrow intercellular spaces within the taste bud, it is expected that the ionic composition of the extracellular fluid surrounding taste cells may be altered significantly by activity. This consideration has led to postulate the existence of glialike cells that could control the microenvironment in taste buds. However, the functional identification of such cells has been so far elusive. By using the patch-clamp technique in voltage-clamp conditions, I identified a new type of cells in the taste buds of the mouse vallate papilla. These cells represented about 30% of cells patched in taste buds and were characterized by a large leakage current. Accordingly, I named them "Leaky" cells. The leakage current was carried by K(+), and was blocked by Ba(2+) but not by tetraethylammonium (TEA). Other taste cells, such as those possessing voltage-gated Na(+) currents and thought to be chemosensory in function, did not express any sizeable leakage current. Consistent with the presence of a leakage conductance, Leaky cells had a low input resistance (approximately 0.25 G Omega). In addition, their zero-current ("resting") potential was close to the equilibrium potential for potassium ions. The electrophysiological analysis of the membrane currents remaining after pharmacological block by Ba(2+) revealed that Leaky cells also possessed a Cl(-) conductance. However, in resting conditions the membrane of these cells was about 60 times more permeable to K(+) than to Cl(-). The resting potassium conductance in Leaky cells could be involved in dissipating rapidly the increase in extracellular K(+) during action potential discharge in chemosensory cells. Thus Leaky cells might represent glialike elements in taste buds. These findings support a model in which specific cells control the chemical composition of intercellular fluid in taste buds.
机译：味蕾是由紧密堆积的专门上皮细胞（称为味细胞）组成的感觉结构。味觉细胞在功能上是异质的，并且它们的很大一部分在化学转导过程中激发动作电位。考虑到味蕾内的狭窄细胞间空间，预期味觉细胞周围的细胞外液的离子组成可被活性显着改变。这种考虑导致推测可以控制味蕾中微环境的胶质样细胞的存在。然而，到目前为止，对此类细胞的功能鉴定尚不清楚。通过在电压钳位条件下使用膜片钳技术，我在小鼠缬草乳头的味蕾中发现了一种新型细胞。这些细胞约占味蕾中修补细胞的30​​％，其特征是泄漏电流大。因此，我将它们命名为“泄漏”单元。泄漏电流由K（+）承载，被Ba（2+）阻止，但未被四乙铵（TEA）阻止。其他味觉细胞，例如那些具有电压门控Na（+）电流并被认为具有化学感应功能的细胞，未表现出任何可观的泄漏电流。与泄漏电导的存在一致，泄漏电池的输入电阻较低（约0.25 G Omega）。另外，它们的零电流（“静止”）电势接近钾离子的平衡电势。 Ba（2+）药理阻滞后残留的膜电流的电生理分析表明，渗漏细胞也具有Cl（-）电导。但是，在静止条件下，这些细胞的膜对K（+）的渗透性比对Cl（-）的渗透性高60倍。渗漏细胞中的静息钾电导可能参与耗散化学感觉细胞动作电位放电过程中细胞外K（+）的迅速增加。因此，泄漏的细胞可能代表味蕾中的胶质样元素。这些发现支持了一种模型，其中特定细胞控制味蕾中细胞间液的化学组成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号