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首页> 外文期刊>Journal of Neuroscience Methods >A miniature CCD video camera for high-sensitivity light measurements in freely behaving animals.
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A miniature CCD video camera for high-sensitivity light measurements in freely behaving animals.

机译：微型CCD摄像机，用于对行为自由的动物进行高灵敏度的光测量。

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摘要

We developed a miniaturized, high-sensitivity camera that can be placed in areas of difficult access in freely behaving animals for neural tissue imaging. The device consists of a charged coupled device (CCD) chip, a coherent image conduit and miniature light emitting diodes (LEDs). An amplifier circuit is constructed on the camera chip and nine wires are attached for external connections. Placement of LEDs around the image conduit perimeter provides dark-field illumination, which increases detection of cellular-related light scattering changes and doubles the depth-of-view over conventional reflectance imaging procedures. The device has been successfully used to record from several deep brain structures, including the ventral medullary surface of sleeping and waking cats. The procedure allows assessment of light scattering changes that result from neural activity or detection of vital dyes to metabolic or voltage-induced activation.
机译：我们开发了一种小型高灵敏度相机，可以将其放置在行为自由的动物难以进入的区域以进​​行神经组织成像。该设备由一个电荷耦合器件（CCD）芯片，一个相干图像导管和微型发光二极管（LED）组成。在相机芯片上构建了一个放大电路，并连接了九根线以进行外部连接。 LED围绕图像导管周边的放置提供了暗视场照明，与传统的反射率成像程序相比，它增加了对与细胞有关的光散射变化的检测，并使景深加倍。该设备已成功用于记录多个深部大脑结构，包括熟睡和醒来的猫的腹髓面。该程序可以评估由于神经活动或检测到重要的染料对代谢或电压诱导的活化而引起的光散射变化。

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