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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Binding and photocleavage of cationic porphyrin-phenylpiperazine hybrids to DNA.
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Binding and photocleavage of cationic porphyrin-phenylpiperazine hybrids to DNA.

机译：阳离子卟啉-苯基哌嗪杂种与DNA的结合和光裂解。

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摘要

The binding properties of cationic porphyrin-phenylpiperazine hybrids to calf thymus (CT) DNA were investigated by using absorption, fluorescence and circular dichroism (CD) spectra, and the apparent affinity binding constants (K(app)) of the porphyrins for CT DNA were determined by using a competition method with ethidium bromide (EB). Intercalation of porphyrin into CT DNA occurred when two phenylpiperazines were introduced at cis position onto the periphery of cationic porphyrin. The photocleavages of pBR322 plasmid DNA by the porphyrins were consistent with the values of K(app). With [porphyrin]/[DNA base pairs] ratio increased, the binding mode tended to be outside binding, and the cleavage abilities of the porphyrins varied. In the presence of sodium azide, a quencher of 1O2, the cleavage of DNA by the porphyrin of intercalation was less inhibited.
机译：通过吸收，荧光和圆二色性（CD）光谱研究了阳离子卟啉-苯基哌嗪杂种与小牛胸腺（CT）DNA的结合特性，卟啉对CT DNA的表观亲和结合常数（K（app））为通过与溴化乙锭（EB）的竞争法测定。当两个苯基哌嗪在顺式位置引入阳离子卟啉外围时，卟啉插入CT DNA中。卟啉对pBR322质粒DNA的光裂解与K（app）的值一致。随着[卟啉] / [DNA碱基对]比例的增加，结合模式趋向于外部结合，并且卟啉的切割能力变化。在叠氮化钠（一种1O2的淬灭剂）的存在下，嵌入的卟啉对DNA的裂解的抑制作用受到抑制。

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