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首页> 外文期刊>Journal of Neuroscience Methods >Patch clamp recording from the intact dorsal root ganglion.
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Patch clamp recording from the intact dorsal root ganglion.

机译：修补钳记录完整的背根神经节。

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摘要

A method for patch-clamp recording from intact dorsal root ganglion (DRG) cells in rat is described. The L4 and L5 DRGs with sciatic nerve attached were excised from rats (10-15 days old) and placed in a recording chamber after removing the ganglion sheath and dissolving the connective tissue with dilute collagenase. The somata of individual cells were exposed by gentle surface cleaning through a perfusion micropipette. Somata were classified as Abeta, Adelta or C based on the cell size and the shape of the action potential (AP). Under current clamp, axonal conduction velocity (CV) was calculated from the distance between a stimulating electrode and the center of the ganglion divided by the latency of the AP elicited by stimulation of the sciatic nerve. CVs ranged from 0.2-0.8 m/s for C cells, 0.8-2.4 for Adelta and 3.2-5.0 for A/beta cells. AP threshold occurred at a significantly more positive potential in C cells than in Adelta and Abeta cells. Under voltage clamp, sodium currents were recorded from C cells. Both TTX-resistant (TTX-R) and TTX-sensitive (TTX-S currents) were demonstrated in the present study. The results demonstrate the feasibility of patch-clamp recording from intact, identified DRG cells in vitro.
机译：描述了一种从大鼠完整的背根神经节（DRG）细胞中膜片钳记录的方法。从大鼠（10-15天大）上切除附有坐骨神经的L4和L5 DRG，并在除去神经节鞘并用稀释的胶原酶溶解结缔组织后将其放置在记录室中。通过灌注微量移液器通过轻柔的表面清洁暴露单个细胞的索玛菌。根据细胞大小和动作电位（AP）的形状，Somata被分类为Abeta，Adelta或C。在电流钳下，通过刺激电极与神经节中心之间的距离除以坐骨神经刺激引起的AP潜伏期来计算轴突传导速度（CV）。 C细胞的CV范围为0.2-0.8 m / s，Adelta的CV范围为0.8-2.4，A / beta细胞的CV范围为3.2-5.0。在C细胞中，AP阈值比Adelta和Abeta细胞具有更高的正电位。在电压钳制下，记录了来自C细胞的钠电流。在本研究中证明了抗TTX（TTX-R）和TTX敏感（TTX-S电流）。结果证明了从完整的，已鉴定出的DRG细胞体外进行膜片钳记录的可行性。

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