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首页> 外文期刊>Journal of Neuroscience Methods >Determination of stereotaxic coordinates for the hippocampus in the domestic pig.
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Determination of stereotaxic coordinates for the hippocampus in the domestic pig.

机译：测定家猪海马的立体定位坐标。

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摘要

In this study a stereotaxic instrument and a stereotaxic procedure based on external skull structures, to be used in prepubertal male Landrace pigs weighing less than 30 kg, is described. The instrument represents an adaptation of the apparatus designed by Marcilloux et al., Brain Res Bull 1989;22:591-597, but we have modified the instrument for stereotaxic procedures based on external skull structures, instead of intracerebral structures necessitating ventriculography (Marcilloux et al., Brain Res Bull 1989;22:591-597). For this reason the U-shaped frame and the ear-bar supports have been changed allowing the three-dimensional placement of the ear-bars into the oblique auditory canals. Firm fixation of the skulls of pigs weighing less than 30 kg, was furthermore secured with modified infraorbital ridges and hard palate pieces. Measurements of distances between external skull structures in animals of the same sex, age and weight showed a negligible variation, thus enabling definition of the horizontal, frontal and sagittal zero planes using external skull structures alone. Stereotaxic coordinates for the hippocampal region of male Landrace pigs weighing 10 kg were then provided and the coordinates from two different levels of the hippocampal region are presented in the text. The reliability of the stereotaxic instrument was finally secured by intrahippocampal injections of ink at predetermined coordinates.
机译：在这项研究中，描述了一种基于外部头骨结构的立体定位仪器和立体定位程序，用于体重小于30 kg的青春期前雄性Landrace猪。该仪器代表了Marcilloux等人（Brain Res Bull 1989; 22：591-597）设计的仪器的改编，但我们已经修改了用于基于外部颅骨结构的立体定位手术的仪器，而不是需要脑室造影的脑内结构（Marcilloux等人等，Brain Res Bull 1989; 22：591-597）。因此，已更改了U形框架和耳杆支架，从而可以将耳杆三维放置在倾斜的耳道中。用改良的眶下和硬the片进一步固定体重不到30公斤的猪的头骨。在相同性别，年龄和体重的动物中，外部颅骨结构之间的距离测量结果可忽略不计，因此仅使用外部颅骨结构就可以定义水平，额骨和矢状零平面。然后提供了体重为10 kg的雄性长白猪的海马区立体坐标，并在文本中显示了来自两个不同水平海马区的坐标。最终，通过在预定坐标下海马内注入墨水来确保立体定位仪器的可靠性。

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