...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neuroscience Methods >Optical monitoring of PO2 changes and simultaneous recording of bioelectric activity in human and animal brain slices.
【24h】

Optical monitoring of PO2 changes and simultaneous recording of bioelectric activity in human and animal brain slices.

机译：光学监测PO2变化并同时记录人和动物脑片中的生物电活动。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

For investigations of hypoxic effects in nervous tissue, brain slices are often used as a model system. This provides the advantage that parameters of the micromilieu, e.g. pH and temperature can easily be controlled and measurements of different data, e.g. bioelectric potentials, ion activities etc. can be performed. It is of special importance that the PO2 the slice preparation is exposed to is equally controlled under these conditions. Therefore, a PO2 monitoring system is needed which provides representative values for the tissue environment. This requirement is fulfilled by an optical PO2 sensing method based on phosphorescence quenching as a function of PO2. Here, the application of this method as adapted for use in in vitro models is described and compared to the polarographic oxygen-sensing method. Both the optical and polarographic methods are comparable regarding accuracy and response time of measurements. Furthermore, both the optical method and electrophysiological measurements can be combined. Lastly, under experimental conditions, neither the phosphorescent dye Palladium-meso-tetra-4-carboxyphenyl-porphine nor the illumination necessary for excitation of the dye influence bioelectric activity of neuronal tissue in vitro. In conclusion, the optical PO2 sensing method presented here provides a tool for reliable and continuous monitoring of PO2 in the immediate environment of brain slice preparations.
机译：为了研究神经组织中的缺氧效应，经常使用脑片作为模型系统。这提供了微环境的参数，例如微孔的参数。可轻松控制pH和温度，并可测量不同的数据，例如可以执行生物电势，离子活性等。特别重要的是，在这些条件下，切片制剂所接触的PO2必须受到同样的控制。因此，需要一种为组织环境提供代表性值的PO2监测系统。通过基于磷光猝灭作为PO2函数的PO2光学传感方法可以满足此要求。在这里，描述了该方法的适用于体外模型的应用，并将其与极谱氧气检测方法进行了比较。光学和极谱法在测量的准确性和响应时间方面都是可比的。此外，可以将光学方法和电生理学测量结合起来。最后，在实验条件下，磷光染料钯-间位-四-4-羧基苯基-卟啉或激发该染料所需的照明都不会影响神经元组织的体外生物电活性。总之，此处介绍的光学PO2传感方法提供了一种在脑切片准备工作的即时环境中可靠且连续地监视PO2的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号