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首页> 外文期刊>Journal of Neuroscience Methods >In vivo predegeneration of peripheral nerves: an effective technique to obtain activated Schwann cells for nerve conduits.
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In vivo predegeneration of peripheral nerves: an effective technique to obtain activated Schwann cells for nerve conduits.

机译：体内周围神经的变性：一种获得活化的神经导管的雪旺细胞的有效技术。

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摘要

In vivo predegeneration of peripheral nerves is presented as a convenient and effective method to obtain activated Schwann cells and an enhanced cell yield following in vitro cultivation. The experiments conducted in rats were aimed at clinical use in gaining Schwann cell suspensions for filling artificial conduits in order to bridge peripheral nerve gaps. The rat sciatic nerve used as a model was transected distally to the spinal ganglia. Predegeneration in vivo was allowed to take place for 1, 2, 3 and 4 days and up to 1, 2 and 3 weeks. The nerve was then resected and prepared for cell cultivation. Schwann cells cultivated from the contralateral untreated nerve served as control. Immunostaining for S100, nerve growth factor receptor and the adhesion molecules N-cadherin and L1 was used to characterize the general state of the cultures. Viability was assessed by fluorescein fluorescence staining, and the proliferation index was determined by bromodeoxyuridine-DNA incorporation. The Schwann cells from predegenerated nerves revealed an increased proliferation rate compared to the control, whereas fibroblast contamination was decreased. Best results were obtained 1 week after predegeneration.
机译：外周神经的体内预变性被认为是获得活化的雪旺细胞并在体外培养后提高细胞产量的方便有效的方法。在大鼠中进行的实验旨在获得用于填充人造导管以弥合周围神经间隙的施旺细胞悬浮液的临床用途。将作为模型的大鼠坐骨神经横切至脊髓神经节。体内发生变性的时间为1、2、3和4天，最多1、2和3周。然后切除神经并准备进行细胞培养。从对侧未处理的神经中培养的雪旺氏细胞用作对照。对S100，神经生长因子受体和粘附分子N-钙黏着蛋白和L1进行免疫染色来表征培养的一般状态。通过荧光素荧光染色评估生存力，并通过溴脱氧尿苷-DNA掺入确定增殖指数。与对照相比，来自变性神经的雪旺细胞显示出增加的增殖速率，而成纤维细胞污染则减少了。变性前1周获得最佳结果。

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