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首页> 外文期刊>Journal of Neuroscience Methods >Validation of a new antiserum directed towards the synthetic c-terminus of the FOS protein in avian species: immunological, physiological and behavioral evidence.
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Validation of a new antiserum directed towards the synthetic c-terminus of the FOS protein in avian species: immunological, physiological and behavioral evidence.

机译：针对禽类中FOS蛋白合成c端的新型抗血清的验证：免疫，生理和行为证据。

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摘要

In the past 10 years, the study of the expression of immediate early genes, such as c-fos, in the brain has become a common method for the identification of brain areas involved in the regulation of specific physiological and behavioral functions. The use of this method in avian species has been limited by the paucity of suitable antibodies that cross-react with the FOS protein in birds. We describe in this paper the preparation of an antibody directed against a synthetic fragment of the protein product of the c-fos gene in chickens (Gallus domesticus). We demonstrate that this new antibody can be used in several avian species to study FOS expression induced by a variety of pharmacological, physiological and behavioral stimuli. Western blot studies indicated that this antibody recognizes a protein of the expected size (47 kDa) but also cross reacts to some extent with proteins of lower molecular weight that share sequence homology with FOS (Fos-related antigens). FOS immunocytochemistry was performed with this antibody in four species of birds in three different laboratories utilizing diverse variants of the immunocytochemical procedure. In all cases the antibody provided a reliable identification of the FOS antigen. The new antibody described here appears to be suitable for the study of FOS expression in several different avian species and situations. It is available in substantial amounts and will therefore make it possible to use FOS expression as a tool to map brain activity in birds as has now been done for several years in mammalian species.
机译：在过去的十年中，研究早期早期基因（例如c-fos）在大脑中的表达已成为鉴定参与调节特定生理和行为功能的大脑区域的常用方法。由于缺乏与禽类中FOS蛋白交叉反应的合适抗体，该方法在禽类中的应用受到了限制。我们在本文中描述了针对鸡（Gallus domesticus）中c-fos基因蛋白产物合成片段的抗体的制备。我们证明了这种新抗体可用于几种禽类，以研究由多种药理，生理和行为刺激诱导的FOS表达。 Western印迹研究表明，该抗体可识别预期大小（47 kDa）的蛋白质，但也会与与FOS（Fos相关抗原）具有序列同源性的较低分子量蛋白质发生交叉反应。在三个不同的实验室中，利用免疫细胞化学程序的各种变体，在四种鸟类中对该抗体进行了FOS免疫细胞化学分析。在所有情况下，该抗体都能可靠地鉴定FOS抗原。此处描述的新抗体似乎适合研究几种不同鸟类和环境中的FOS表达。它可以大量使用，因此将FOS表达用作绘制鸟类大脑活动图谱的工具成为可能，就像在哺乳动物物种中已经使用了数年。

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