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首页> 外文期刊>Journal of Neuroscience Methods >Methods to evaluate functional nerve recovery in adult rats: walking track analysis, video analysis and the withdrawal reflex.
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Methods to evaluate functional nerve recovery in adult rats: walking track analysis, video analysis and the withdrawal reflex.

机译:评估成年大鼠功能神经恢复的方法:步行轨迹分析,视频分析和退缩反射。

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The aim of this study was to compare different methods for the evaluation of functional nerve recovery. Three groups of adult male Wistar rats were studied. In group A, a 12-mm gap between nerve ends was bridged by an autologous nerve graft; in rats of group B we performed a crush lesion of the sciatic nerve and group C consisted of non-operated control rats. The withdrawal reflex, elicited by an electric stimulus, was used to evaluate the recovery of sensory nerve function. To investigate motor nerve recovery we analyzed the walking pattern. Three different methods were used to obtain data for footprint analysis: photographic paper with thickened film developer on the paws, normal white paper with finger paint, and video recordings. The footprints were used to calculate the sciatic function index (SFI). From the video recordings, we also analyzed stepcycles. The withdrawal reflex is a convenient and reproducible test for the evaluation of global sensory nerve recovery. Recording walking movements on video and the analysis of footplacing is a perfect although time-consuming method for the evaluation of functional aspects of motor nerve recovery.
机译:这项研究的目的是比较评估功能神经恢复的不同方法。研究了三组成年雄性Wistar大鼠。在A组中,通过自体神经移植物弥合了神经末梢之间的12mm间隙。在B组的大鼠中,我们对坐骨神经进行了压迫性损伤,C组由未手术的对照大鼠组成。由电刺激引起的退出反射被用于评估感觉神经功能的恢复。为了研究运动神经的恢复,我们分析了步行模式。使用三种不同的方法来获取用于足迹分析的数据:在脚掌上带有增稠膜显影剂的相纸,在手指上涂有颜料的普通白纸以及录像。足迹用于计算坐骨神经功能指数(SFI)。从录像中,我们还分析了步骤周期。退缩反射是评估整体感觉神经恢复的便捷且可重复的测试。在视频上记录步行运动并进行脚步分析是一种完美的方法,尽管它是评估运动神经恢复功能方面的耗时方法。

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