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首页> 外文期刊>Journal of Neuroscience Methods >A novel mouse brain slice preparation of the hippocampo-accumbens pathway.
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A novel mouse brain slice preparation of the hippocampo-accumbens pathway.

机译：海马-accumbens途径的新型小鼠脑切片制备。

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The nucleus accumbens (NAc) is an important component of circuitry that underlies reward related behaviors and the rewarding properties of drugs of abuse. Glutamatergic afferents to the nucleus are critical for its normal function and for behaviors related to drug addiction. An angled, sagittal mouse brain slice preparation has been designed to facilitate concurrent stimulation of two major glutamatergic afferent pathways to the nucleus accumbens. Medium spiny neurons at the medial core/shell boundary of the accumbens were depolarized by stimulation of either hippocampal or limbic cortical afferents through activation of AMPA-type glutamate receptors. High frequency but not low frequency stimulation of hippocampal afferents depolarized medium spiny neurons to a membrane potential that resembled the up state observed upon high frequency stimulation in vivo. The magnitude of the membrane depolarization was positively correlated with the amplitude of the stimulus-evoked EPSP. Concurrent stimulation of hippocampal and limbic cortical afferents at theta frequency selectively induced a long-term depression (LTD) in the magnitude of stimulus-evoked EPSPs on the hippocampal afferent only. These data suggest that this brain slice preparation can be used to study mechanisms underlying synaptic plasticity at two of the critical glutamatergic afferent synapses in the nucleus accumbens as well as characterizing potential interactions between afferents. Additionally, LTD at hippocampo-accumbens synapses can be induced at a stimulus frequency known to support reinstatement of drug seeking behavior.
机译：伏伏核（NAc）是电路的重要组成部分，是奖励相关行为和滥用药物的奖励特性的基础。谷氨酸能传入核对于其正常功能和与药物成瘾有关的行为至关重要。已设计了成角度的矢状小鼠脑片制剂，以促进同时刺激伏伏核的两个主要谷氨酸能传入途径。通过激活AMPA型谷氨酸受体刺激海马或边缘皮质传入神经，使伏隔肌内侧核/壳边界的中棘神经元去极化。海马传入体的高频刺激而非低频刺激使中多刺神经元去极化，使其膜电位类似于体内高频刺激时观察到的向上状态。膜去极化的幅度与刺激诱发的EPSP的幅度呈正相关。以theta频率同时刺激海马和边缘皮质传入神经选择性地诱发了仅在海马传入刺激上引起的EPSPs幅度的长期抑制（LTD）。这些数据表明，这种脑切片的制备可用于研究伏伏核中两个关键谷氨酸能传入突触的突触可塑性的基础机制，以及表征传入突触之间的潜在相互作用。另外，在海马-突触突触处的LTD可以以已知支持刺激寻药行为恢复的刺激频率被诱导。

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