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首页> 外文期刊>Journal of Neuroscience Methods >Neuronal development and migration in zebrafish hindbrain explants.
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Neuronal development and migration in zebrafish hindbrain explants.

机译：斑马鱼后脑外植体中神经元的发育和迁移。

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摘要

The zebrafish embryo is an excellent system for studying dynamic processes such as cell migration during vertebrate development. Dynamic analysis of neuronal migration in the zebrafish hindbrain has been hampered by morphogenetic movements in vivo, and by the impermeability of embryos. We have applied a recently reported technique of embryo explant culture to the analysis of neuronal development and migration in the zebrafish hindbrain. We show that hindbrain explants prepared at the somitogenesis stage undergo normal morphogenesis for at least 14 h in culture. Importantly, several aspects of hindbrain development such as patterning, neurogenesis, axon guidance, and neuronal migration are largely unaffected, inspite of increased cell death in explanted tissue. These results suggest that hindbrain explant culture can be employed effectively in zebrafish to analyze neuronal migration and other dynamic processes using pharmacological and imaging techniques.
机译：斑马鱼胚胎是研究脊椎动物发育过程中细胞迁移等动态过程的出色系统。斑马鱼后脑神经元迁移的动态分析受到体内形态发生运动和胚胎的不渗透性的阻碍。我们已将最近报道的胚胎外植体培养技术应用于斑马鱼后脑神经元发育和迁移的分析。我们表明，在体细胞发生阶段制备的后脑外植体在培养中至少经历了14 h的正常形态发生。重要的是，尽管外植组织中细胞死亡的增加，但后脑发育的几个方面（例如模式，神经发生，轴突引导和神经元迁移）基本上不受影响。这些结果表明，使用药理学和成像技术，后脑外植体培养可以有效地用于斑马鱼中，以分析神经元迁移和其他动态过程。

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