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首页> 外文期刊>Journal of Neuroscience Methods >Dextran backfill tracers combined with Lucifer yellow injections for neuroanatomic studies of the leech head ganglion.
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Dextran backfill tracers combined with Lucifer yellow injections for neuroanatomic studies of the leech head ganglion.

机译：右旋糖酐回填示踪剂结合路西法黄注射液用于水ech头神经节的神经解剖学研究。

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摘要

Several neuronal tracing substances were applied to the cut ends of leech cephalic nerves and the resulting backfills into the subesophageal ganglion (sbEG) were mapped. A 12 h incubation in 3 kDa dextrans conjugated either to a fluorochrome or to biotin (subsequently tagged with peroxidase) was satisfactory. In separate experiments, possible targets of cephalic nerve afferents (R3 Retzius neurons) were injected with Lucifer Yellow (LY) to visualize their projections. Comparison of the LY-R3 Retzius neuron map with that of the dextran-backfilled D1 nerve revealed extensive overlap in the sbEG. Experiments were performed combining the two protocols, confirming this observation. Moreover, confocal microscopy placed D1 nerve processes in close proximity to R3 Retzius neuron processes, suggesting that they could make synaptic contact with one another in the sbEG. With modifications, this method could be used to identify such contacts using electron microscopy.
机译：将几种神经元示踪物质施加到水ech头神经的切开端，并将产生的回填物映射到食管下神经节（sbEG）。在与荧光染料或生物素（随后用过氧化物酶标记）偶联的3 kDa葡聚糖中孵育12小时是令人满意的。在单独的实验中，向头颅神经传入神经（R3 Retzius神经元）的可能目标注射了路西法黄（LY），以可视化它们的投影。 LY-R3 Retzius神经元图谱与右旋糖酐回填的D1神经图谱的比较显示sbEG广泛重叠。结合两种方案进行了实验，证实了这一观察结果。此外，共聚焦显微镜将D1神经过程置于R3 Retzius神经元过程的附近，这表明它们可以使sbEG中的突触彼此接触。经过修改，该方法可用于通过电子显微镜识别这种接触。

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