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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Adsorption of synthetic homo- and hetero-oligodeoxynucleotides onto highly oriented pyrolytic graphite: Atomic force microscopy characterization
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Adsorption of synthetic homo- and hetero-oligodeoxynucleotides onto highly oriented pyrolytic graphite: Atomic force microscopy characterization

机译：合成的均聚和杂聚寡脱氧核苷酸在高度定向的热解石墨上的吸附：原子力显微镜表征

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摘要

DNA adsorption on electrode surfaces is of fundamental interest for the development of DNA-based biosensors. The free adsorption of 10-mer synthetic oligodeoxynucleotides (ODNs) onto highly oriented pyrolytic graphite (HOPG) surfaces was studied using Magnetic AC mode atomic force microscopy (MAC Mode AFM). The mechanism of interaction of nucleic acids with carbon electrode surfaces was elucidated, using 10-mer synthetic homo- and hetero-ODNs sequences of known base sequences, because they allow clear interpretation of the experimental data. AFM images in air revealed different adsorption patterns and degree of HOPG surface coverage for the ODNs, and correlation with the individual structure and base sequence of each ODN molecule will be presented. The results demonstrated that the hydrophobic interactions with the HOPG hydrophobic surface explain the main adsorption mechanism, although other effects such as electrostatic and Van der Waals interactions may contribute to the free adsorption process. The ODNs interacted differently with the H OPG surface, according to the ODN sequence hydrophobic characteristics, being directly depending on the molecular mass, the hydrophobic character of the individual bases and on the secondary structure of the molecule. The importance of the type of base existent at the ODN chain extremities on the adsorption process was investigated and different adsorption patterns were obtained with ODN sequences composed by the same group of bases aligned in a different order. (c) 2006 Elsevier B.V. All rights reserved.
机译：DNA吸附在电极表面上对于开发基于DNA的生物传感器至关重要。使用磁性AC模式原子力显微镜（MAC模式AFM）研究了10-mer合成寡聚脱氧核苷酸（ODN）在高度定向的热解石墨（HOPG）表面上的自由吸附。使用已知碱基序列的10-mer合成的同源和异源ODN序列，阐明了核酸与碳电极表面相互作用的机制，因为它们可以清楚地解释实验数据。空气中的AFM图像揭示了ODN的不同吸附模式和HOPG表面覆盖程度，并将显示与每个ODN分子的个体结构和碱基序列的相关性。结果表明，与HOPG疏水表面的疏水相互作用解释了主要的吸附机理，尽管静电和范德华相互作用等其他作用也可能有助于自由吸附过程。根据ODN序列的疏水特性，ODN与H OPG表面的相互作用不同，这直接取决于分子质量，各个碱基的疏水特性以及分子的二级结构。研究了ODN链末端存在的碱基类型对吸附过程的重要性，并使用以不同顺序排列的同一组碱基组成的ODN序列获得了不同的吸附模式。 （c）2006 Elsevier B.V.保留所有权利。

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