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首页> 外文期刊>Journal of Neuroscience Methods >Utilizing X-irradiation to selectively eliminate neural stem/progenitor cells from neurogenic regions of the mammalian brain.
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Utilizing X-irradiation to selectively eliminate neural stem/progenitor cells from neurogenic regions of the mammalian brain.

机译：利用X射线辐射从哺乳动物大脑的神经源性区域选择性清除神经干/祖细胞。

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Neural stem/progenitor cells residing in the mammalian CNS provide a potential endogenous source for replenishing neurons that are lost due to aging, trauma or disease. However, little is known about their functional potential due to the lack of methodologies that allow for the reproducible alteration of stem cell numbers in vivo. Accordingly, we describe a methodology that utilizes targeted X-irradiation to experimentally generate neural stem/progenitor cell-depleted rat models. We show that, by virtue of their mitotic activity, proliferating neural stem/progenitor cells can be selectively eliminated from either the subventricular zone (SVZ) or dentate gyrus of a rat by treating it to an (unilateral or bilateral) exposure of X-irradiation. Utilizing BrdU incorporation, it was found that a single 15 gray (Gy) exposure to the SVZ resulted in the elimination of 85% of the proliferating cell population for up to 3 months. Immunohistochemistry, ultrastructural analysis and proteomics were employed to confirm that the cells eliminated following X-irradiation were neural stem/progenitor cells. Similar depletions of the stem/progenitor cell population in the dentate gyrus were achieved by targeting the hippocampus with a single 15Gy exposure. The reproducibility, versatility and ease of generation make these experimental animal models a valuable tool to aid in our understanding of the properties and functions of neural stem/progenitor cells.
机译：驻留在哺乳动物CNS中的神经干/祖细胞为补充因衰老，创伤或疾病而丧失的神经元提供了潜在的内源性来源。然而，由于缺乏使体内干细胞数目可再现地改变的方法，对它们的功能潜力知之甚少。因此，我们描述了一种方法，该方法利用靶向的X射线辐射来实验性地生成神经干/祖细胞耗竭的大鼠模型。我们表明，凭借其有丝分裂活性，可以通过对大鼠的单侧或双侧X线照射进行处理，从大鼠的脑室下区（SVZ）或齿状回中选择性地消除增殖性神经干/祖细胞。 。利用BrdU掺入，发现对SVZ的一次15 gray（Gy）暴露导致最多3个月消除了85％的增殖细胞群。免疫组织化学，超微结构分析和蛋白质组学被用来确认X射线照射后消除的细胞是神经干/祖细胞。通过单次15Gy暴露靶向海马，就可以实现齿状回中干/祖细胞群的类似耗竭。可再现性，多功能性和易于生成使这些实验动物模型成为有价值的工具，有助于我们了解神经干/祖细胞的特性和功能。

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