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首页> 外文期刊>Journal of Neuroscience Methods >Demonstration of vessels in CNS and other organs by AMG silver enhancement of colloidal gold particles dispersed in gelatine.
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Demonstration of vessels in CNS and other organs by AMG silver enhancement of colloidal gold particles dispersed in gelatine.

机译：通过AMG银增强分散在明胶中的胶体金颗粒，证明CNS和其他器官中的血管。

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摘要

We present a new autometallographic technique for demonstrating vessels and other small cavities at light microscopy (LM) and electron microscopy (EM) levels. It is possible to obtain detailed knowledge of the 3-D appearance of the vascular system by exchanging blood with a 40 degrees C, 8% gelatine solution containing colloidal gold particles (gold gelatine solution, GGS) and ensuing silver enhancement of the gold particles by autometallography (AMG). The GGS-AMG technique demonstrates the vascular system as a dark web that can be studied in cryostat, vibratome, methacrylate, paraffin and Epon sections at all magnifications. The infused GGS becomes increasingly viscous and finally becomes rigid when the temperature falls below 20 degrees C. An additional advantage of this technique is the fact that none of the tested counterstains or immunotechniques interfere with this AMG approach. The GGS-AMG technique is demonstrated on rat brains but can be applied to any organ. We believe that the present technique is valuable for both experimental studies and routine pathology.
机译：我们提出了一种新的自动金相技术，用于在光学显微镜（LM）和电子显微镜（EM）的水平上展示血管和其他小腔体。通过与含有胶体金颗粒的40摄氏度，8％明胶溶液（金明胶溶液，GGS）交换血液，并通过使金颗粒的银增强，可以获得有关血管系统3-D外观的详细知识。自动金相学（AMG）。 GGS-AMG技术将血管系统显示为暗网，可以在所有放大倍数下的低温恒温器，振动刀，甲基丙烯酸酯，石蜡和Epon切片中进行研究。注入的GGS变得越来越粘稠，并在温度降至20摄氏度以下时最终变得坚硬。该技术的另一个优势是，没有一种经测试的复染色或免疫技术会干扰该AMG方法。 GGS-AMG技术已在大鼠脑部得到证实，但可以应用于任何器官。我们认为，本技术对于实验研究和常规病理学都是有价值的。

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