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首页> 外文期刊>Journal of Neuroscience Methods >Reverse phase protein microarray technology in traumatic brain injury.
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Reverse phase protein microarray technology in traumatic brain injury.

机译：反相蛋白微阵列技术在颅脑外伤中的应用。

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摘要

Antibody based, high throughput proteomics technology represents an exciting new approach in understanding the pathobiologies of complex disorders such as cancer, stroke and traumatic brain injury. Reverse phase protein microarray (RPPA) can complement the classical methods based on mass spectrometry as a high throughput validation and quantification method. RPPA technology can address problematic issues, such as sample complexity, sensitivity, quantification, reproducibility and throughput, which are currently associated with mass spectrometry-based approaches. However, there are technical challenges, predominantly associated with the selection and use of antibodies, preparation and representation of samples and with analyzing and quantifying primary RPPA data. Here we present ways to identify and overcome some of the current issues associated with RPPA. We believe that using stringent quality controls, improved bioinformatics analysis and interpretation of primary RPPA data, this method will significantly contribute in generating new level of understanding about complex disorders at the level of systems biology.
机译：基于抗体的高通量蛋白质组学技术代表了一种令人振奋的新方法，可用于理解复杂疾病（例如癌症，中风和脑外伤）的病理学。反相蛋白质微阵列（RPPA）可以作为高通量验证和定量方法，对基于质谱的经典方法进行补充。 RPPA技术可以解决问题问题，例如样品复杂性，灵敏度，定量，重现性和通量，这些问题目前与基于质谱的方法有关。但是，存在技术挑战，主要与抗体的选择和使用，样品的制备和表示以及与分析和量化主要RPPA数据有关。在这里，我们介绍识别和克服与RPPA相关的一些当前问题的方法。我们相信，使用严格的质量控制，改进的生物信息学分析和RPPA原始数据的解释，该方法将在系统生物学的水平上极大地促进对复杂疾病的新认识。

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