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首页> 外文期刊>Journal of Neuroscience Methods >Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires.
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Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires.

机译：可溶性因子对胶衣微丝表面胶质细胞反应性的影响。

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A basal lamina gel preparation was incorporated into a modified neuroinflammation cell culture model to test the system as a characterization tool for surface-modified microwires. The extent of gliosis at the surface of gel-coated microwires was quantified in response to titrating the cell culture with a number of soluble factors reported to be involved in reactive gliosis. Positive control conditions (1% FBS, 10 ng/ml bFGF, Neural Basal medium with B27 supplement) induced a layer of GFAP-expressing astrocytes to accumulate on all gel-coated microwires. Serum, inflammatory cytokines IL-1alpha and IL-1beta and the neural progenitor cell (NPC) proliferating growth factors bFGF and PDGF all increased the number of reactive cells on the gel, in agreement with their reported roles in cell activation, migration and proliferation at the site of injury. Technically, this study shows that a fetal neuron-glia culture system is well suited for characterizing the impact of electrode coatings designed to mitigate implant-associated gliosis, and for screening the effect of multiple soluble factors that promote and/or inhibit implant-associated gliosis. Scientifically, this study points to essential roles of serum and inflammatory factors to induce NPC activation and migration to the site of injury, where growth factors like bFGF and PDGF induce proliferation of cells that will eventually form the glial scar.
机译：将基础薄层凝胶制剂掺入改良的神经炎症细胞培养模型中，以测试该系统作为表面修饰的微丝的表征工具。响应于用许多据报道与反应性神经胶质细胞增生有关的可溶性因子滴定细胞培养物，对涂有凝胶的微丝表面的神经胶质细胞化程度进行了定量。阳性对照条件（1％FBS，10 ng / ml bFGF，带有B27补充剂的神经基础培养基）诱导表达GFAP的星形胶质细胞层积聚在所有涂有凝胶的微丝上。血清，炎性细胞因子IL-1alpha和IL-1beta以及神经祖细胞（NPC）增殖生长因子bFGF和PDGF均增加了凝胶上反应性细胞的数量，这与它们在细胞活化，迁移和增殖中所报告的作用相一致。受伤的地方。从技术上讲，这项研究表明，胎儿神经胶质细胞培养系统非常适合表征旨在减轻与植入物相关的神经胶质形成的电极涂层的影响，并筛选促进和/或抑制与植入物相关的神经胶质形成的多种可溶性因子的作用。 。从科学上讲，这项研究指出了血清和炎性因子在诱导NPC活化和迁移到损伤部位中的重要作用，其中生长因子（如bFGF和PDGF）诱导细胞增殖，最终形成神经胶质瘢痕。

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