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首页> 外文期刊>Journal of Neuroscience Methods >A novel, non-invasive method of respiratory monitoring for use with stereotactic procedures
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A novel, non-invasive method of respiratory monitoring for use with stereotactic procedures

机译：一种新颖的，无创的呼吸监测方法，用于立体定向手术

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Accurate monitoring of respiration is often needed for neurophysiological studies, as either a dependent experimental variable or an indicator of physiological state. Current options for respiratory monitoring of animals held in a stereotaxic frame include EMG recordings, pneumotachograph measurements, inductance-plethysmography, whole-body plethysmography (WBP), and visual monitoring. While powerful, many of these methods prevent access to the animal's body, interfere with experimental manipulations, or require deep anesthesia and additional surgery. For experiments where these issues may be problematic, we developed a non-invasive method of recording respiratory parameters specifically for use with animals held in a stereotaxic frame. This system, ventilation pressure transduction (VPT), measures variations in pressure at the animal's nostril from inward and outward airflow during breathing. These pressure changes are detected by a sensitive pressure transducer, then filtered and amplified. The output is an analog signal representing each breath. VPT was validated against WBP using 10% carbon dioxide and systemic morphine (4. mg/kg) challenges in lightly anesthetized animals. VPT accurately represented breathing rate and tidal volume changes under both baseline and challenge conditions. This novel technique can therefore be used to measure respiratory rate and relative tidal volume when stereotaxic procedures are needed for neuronal manipulations and recording.
机译：神经生理学研究经常需要对呼吸进行准确的监测，作为相关的实验变量或生理状态的指标。用于对处于立体定向框架中的动物进行呼吸监视的当前选项包括EMG记录，气动描记器测量，电感体积描记法，全身体积描记法（WBP）和视觉监视。这些方法虽然功能强大，但它们阻止动物进入动物体内，干扰实验操作或需要深度麻醉和其他手术。对于这些问题可能有问题的实验，我们开发了一种非侵入性的方法来记录呼吸参数，专门用于在立体定位框架中的动物。该系统称为通气压力转换（VPT），用于测量呼吸过程中动物的鼻孔内向和外向气流中压力的变化。这些压力变化由灵敏的压力传感器检测，然后过滤和放大。输出是代表每次呼吸的模拟信号。在轻度麻醉的动物中，使用10％的二氧化碳和全身性吗啡（4. mg / kg）激发，对VPT进行了针对WBP的验证。 VPT准确地表示了基线和挑战条件下的呼吸速率和潮气量变化。因此，当需要进行神经元操作和记录的立体定向手术时，可以使用这种新技术来测量呼吸频率和相对潮气量。

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