• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >The stability of triplex DNA is affected by the stability of the underlying duplex.
【24h】

The stability of triplex DNA is affected by the stability of the underlying duplex.

机译：三链DNA的稳定性受下面的双链体的稳定性影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have studied the formation of DNA triple helices in different sequence contexts and show that, for the most stable triplexes, their apparent stability is affected by the stability of the underlying duplex. For a 14-mer parallel triplex-forming oligonucleotide (generating C(+).GC and T.AT triplets) at pH 5.0 the T(m) is more than 10 degrees C lower with an intermolecular 14-mer duplex target, than it is with an intramolecular duplex, or one which is flanked by 6 GC base pairs at either end. A similar effect is seen with triplex-forming oligonucleotides that contain stabilising analogues, for which the T(m) is higher for an intramolecular than an intermolecular duplex target. These results suggest that the use of simple intermolecular duplex targets may underestimate the triplex stabilisation that is produced by some nucleotide analogues.
机译：我们研究了在不同序列背景下DNA三重螺旋的形成，并表明，对于最稳定的三重螺旋，其表观稳定性受基础双链体的稳定性影响。对于pH 5.0的14-mer平行三链体形成寡核苷酸（产生C（+）。GC和T.AT三联体），分子间14-mer双链体靶标的T（m）比其低10摄氏度以上。带有分子内双链体，或者在任一端侧接6个GC碱基对。用含有稳定类似物的三链体形成寡核苷酸可以看到类似的效果，对于它们，分子内T（m）高于分子间双链体靶标。这些结果表明，使用简单的分子间双链体靶标可能会低估某些核苷酸类似物产生的三链体稳定性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号