• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Self assembly of poly(o-methoxy aniline) with RNA and RNA/DNA hybrids: physical properties and conformational change of poly(o-methoxy aniline).
【24h】

Self assembly of poly(o-methoxy aniline) with RNA and RNA/DNA hybrids: physical properties and conformational change of poly(o-methoxy aniline).

机译：聚（邻甲氧基苯胺）与RNA和RNA / DNA杂化物的自组装：聚（邻甲氧基苯胺）的物理性质和构象变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Biomolecular hybrids of a conducting polymer [poly(o-methoxy aniline) (POMA)] and RNA are prepared at the three different compositions by mixing aqueous solutions of diethyl, 2-hydroxy ethyl, ammonium salt of RNA (type IX from Torula Yeast) and POMA (ES, emeraldine sa doping level [Cl]/[N]=0.52). A slow increase of pH up to 30 h of aging occurs in the mixture till it levels up. The TEM micrographs indicate a fibrillar network structure in all the hybrid compositions (POMA: RNA=1:3, 1:1, 3:1, by weight). In the complexes three types of supramolecular interactions, viz. (i) electrostatic, (ii) H-bonding and (iii) pi-pi interactions, are evident from the FTIR spectroscopy. The CD spectra indicate a small distortion of A-RNA conformation towards its B form during the hybrid formation. Time and temperature dependent UV-vis spectral studies indicate a slow red shift of the pi-band to polaron band transition peak (lambda(max)) for the uncoiling of the POMA (P) chain on the RNA (R) surface. The repulsive interaction between the radical cations of POMA (ES) absorbed on the RNA surface is attributed to the conformational change causing the uncoiling of POMA chain. UV-vis spectral study indicates that the uncoiling and attachment of POMA on RNA surface is much faster than that on DNA (D). In POMA-RNA-DNA (PRD) hybrid solutions slower red shift of lambda(max) indicates more disordered array of the phosphate groups than that in PR and PD systems. The conductivity values of the PR hybrids (10(-)(6) S/cm(-1)) are three orders higher than that of RNA, rendering the PR hybrids to be useful for fabricating good biosensors. In the PRD hybrids conductivity decreases by two orders than those of PR and PD hybrids suggesting a disorder arrangement of POMA chains in the PRD hybrids. The I-V characteristic curves of the PR and PRD hybrids indicate a semiconducting nature of the hybrids.
机译：通过混合RNA的二乙基，2-羟乙基，铵盐水溶液（来自Torula Yeast的IX型），在三种不同的组成下制备导电聚合物[聚（邻甲氧基苯胺）（POMA）]和RNA的生物分子杂化物。和POMA（ES，翡翠盐;掺杂水平[Cl] / [N] = 0.52）。在混合物中，pH值的缓慢升高直至老化30小时，直到pH值升高为止。 TEM显微照片显示了所有杂种组合物中的原纤维网络结构（POMA：RNA = 1：3、1：1、3：1，按重量计）。在复合物中，三种类型的超分子相互作用，即。 （i）静电，（ii）H键和（iii）pi-pi相互作用从FTIR光谱中可以明显看出。 CD光谱表明，在杂种形成过程中，A-RNA构象朝着其B形式的微小变形。与时间和温度有关的UV-vis光谱研究表明，对于RNA（R）表面上POMA（P）链的解绕，π带向极化子带跃迁峰（lambda（max））的缓慢红移。 RNA表面吸收的POMA（ES）自由基阳离子之间的排斥相互作用归因于构象变化，导致POMA链解开。紫外可见光谱研究表明，POMA在RNA表面的解卷和附着比在DNA（D）上的快得多。在POMA-RNA-DNA（PRD）混合溶液中，lambda（max）的红移较慢，表明磷酸基团的排列比PR和PD系统中的排列更混乱。 PR杂种的电导率值（10（-）（6）S / cm（-1））比RNA高3个数量级，这使PR杂种可用于制造良好的生物传感器。在PRD杂种中，电导率比PR和PD杂种降低了两个数量级，这表明PRD杂种中POMA链的排列无序。 PR和PRD杂种的I-V特性曲线表明了杂种的半导体性质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号