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首页> 外文期刊>Journal of Neuroscience Research >Expression of the myelin and oligodendrocyte progenitor marker sulfatide in neurons and astrocytes of adult rat brain.
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Expression of the myelin and oligodendrocyte progenitor marker sulfatide in neurons and astrocytes of adult rat brain.

机译：成年大鼠脑神经元和星形胶质细胞中髓磷脂和少突胶质祖细胞标记物硫的表达。

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Sulfatide is a myelin component of the central (CNS) and peripheral nervous system (PNS) and is used extensively to identify oligodendrocyte progenitor cells. We have explored sulfatide expression in CNS gray matter (cerebellum, cerebral cortex, and hippocampus) and the PNS in adult rats using an anti-sulfatide antibody (Sulph I) and confocal microscopy. Biochemical analyses revealed two Sulph I antigens, sulfatide and seminolipid; sulfatide was present at about five times higher concentration, and the affinity of Sulph I for sulfatide was 2.5 times higher than that for seminolipid. Thus sulfatide was considered the dominant antigen. We found Sulph I immunostaining, in addition to that in myelinated areas in subpopulations of astrocytes and neurons. Astrocyte Sulph I staining was localized to the cell bodies and in some cases also to the processes. In the cerebellum, some Sulph I-positive astrocytes corresponded to Golgi epithelial cell bodies. We also found Sulph I staining in neuronal cell bodies, which in some neurons was clearly localized to the cytoplasm and in others to the nuclear membrane. Sulph I immunostaining in the PNS was located in the myelin sheath and paranodal end segments. These results demonstrate the expression of sulfatide in cell types other than oligodendrocytes and Schwann cells, showing that sulfatide is not a selective marker for adult oligodendrocyte progenitor cells. Moreover, these findings show that sulfatide is localized also to intracellular compartments and indicate that other roles of sulfatide in astrocytes and neurons, compared to myelin, might be considered.
机译：硫化物是中枢神经系统（CNS）和周围神经系统（PNS）的髓磷脂成分，广泛用于鉴定少突胶质细胞祖细胞。我们已经使用抗硫化物抗体（Sulph I）和共聚焦显微镜研究了成年大鼠中枢神经系统灰质（小脑，大脑皮层和海马）和PNS中硫化物的表达。生化分析表明，有两种Sulph I抗原，即硫化物和半脂质。硫化物的浓度高约5倍，并且Sulph I对硫化物的亲和力比对半脂质高2.5倍。因此，硫脂被认为是主要抗原。我们在星形胶质细胞和神经元亚群的髓鞘区域中发现了Sulph I免疫染色。星形胶质细胞Sulph I染色定位于细胞体，在某些情况下还定位于过程。在小脑中，一些Sulph I阳性星形胶质细胞对应于高尔基体上皮细胞体。我们还在神经元细胞体中发现了Sulph I染色，在某些神经元中，Sulph I染色明显地定位于细胞质，而另一些则定位于核膜。 PNS中的Sulph I免疫染色位于髓鞘和淋巴结末端。这些结果证明了脂多糖在少突胶质细胞和雪旺氏细胞以外的细胞类型中的表达，表明硫酸脂不是成年少突胶质细胞祖细胞的选择性标记。此外，这些发现表明，硫酸脂也定位于细胞内区室，并表明与髓磷脂相比，硫酸脂在星形胶质细胞和神经元中的其他作用也可以考虑。

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