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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Chromophoric dissolved organic matter and microbial enzymatic activity. A biophysical approach to understand the marine carbon cycle
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Chromophoric dissolved organic matter and microbial enzymatic activity. A biophysical approach to understand the marine carbon cycle

机译：发色溶解的有机物和微生物的酶活性。了解海洋碳循环的生物物理方法

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This study reports the first information on extracellular enzymatic activity (EEA) combined with a study of DOM dynamics at the Arno River mouth. DOM dynamics was investigated from both a quantitative (dissolved organic carbon, DOC) and a qualitative (absorption and fluorescence of chromophoric DOM, CDOM) perspective. The data here reported highlight that the Arno River was an important source of both DOC and CDOM for this coastal area. CDOM optical properties suggested that terrestrial DOM did not undergo simple dilution at the river mouth but, other physical-chemical and biological processes were probably at work to change its molecular characteristics. This observation was further supported by the "potential" enzymatic activity of p-glucosidase (BG) and leucine aminopeptidase (LAP). Their Vmax values were markedly higher in the river water than in the seawater and their ratio suggested that most of the DOM used by microbes in the Arno River was polysaccharide-like, while in the seawater it was mainly protein-like.
机译：这项研究报告了有关细胞外酶活性（EEA）的第一个信息，并结合了对Arno河口DOM动力学的研究。从定量（溶解有机碳，DOC）和定性（发色DOM，CDOM的吸收和荧光）角度研究了DOM动力学。报告的数据突出表明，阿尔诺河是该沿海地区DOC和CDOM的重要来源。 CDOM的光学性质表明，陆地DOM在河口处没有经过简单的稀释，但是其他物理化学和生物过程可能正在起作用以改变其分子特性。 p-葡萄糖苷酶（BG）和亮氨酸氨基肽酶（LAP）的“潜在”酶促活性进一步支持了这一观察结果。它们的Vmax值在河水中明显高于海水，并且它们的比率表明，阿尔诺河中微生物使用的大多数DOM是多糖样的，而在海水中主要是蛋白样的。

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