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Resonance energy transfer study of peptide-lipid complexes.

机译:肽-脂质复合物的共振能量转移研究。

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摘要

Resonance energy transfer involving tryptophan as a donor and anthrylvinyl-labeled phosphatidylcholine (AV-PC), 3-methoxybenzanthrone (MBA) and 8-anilino-1-naphthalene sulfonic acid (ANS) as acceptors has been examined to obtain information on the structure of peptide-lipid systems consisting of 18A or Ac-18A-NH(2) peptides and large unilamellar phosphatidylcholine vesicles. The lower and upper limits for the tryptophan distance from the bilayer midplane have been assessed in terms of the models of energy transfer in two-dimensional systems, taking into account orientational effects. Evidence for the existence of preferential orientations of Ac-18A-NH(2) with respect to the lipid-water interface has been obtained.
机译:研究了色氨酸作为供体和蒽乙烯基标记的磷脂酰胆碱(AV-PC),3-甲氧基苯并蒽酮(MBA)和8-苯胺基-1-萘磺酸(ANS)作为受体的共振能量转移,以获取有关结构的信息肽-脂质系统,由18A或Ac-18A-NH(2)肽和大型单层磷脂酰胆碱囊泡组成。距双层中平面的色氨酸距离的上限和下限已根据二维系统中的能量转移模型进行了评估,同时考虑了定向效应。已经获得有关Ac-18A-NH(2)相对于脂质-水界面的优先取向的证据。

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