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首页> 外文期刊>Journal of Molluscan Studies >MAPPING OF RIBOSOMAL DNA AND (TTAGGG)_n TELOMERIG SEQUENCE BY FISH IN THE BIVALVE PATINOPECTEN TESSOENSIS (JAY, 1857)
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MAPPING OF RIBOSOMAL DNA AND (TTAGGG)_n TELOMERIG SEQUENCE BY FISH IN THE BIVALVE PATINOPECTEN TESSOENSIS (JAY, 1857)

机译：鱼类在双壳类百日咳TESSOENSIS中映射核糖体DNA和（TTAGGG）_n端粒序列（1857年1月）

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摘要

The chromosome set of Patinopectenyessoensis (Jay, 1857) was characterized using Giemsa staining, DAPI staining and fluorescence in situ hybridization (FISH) with three repetitive DNA probes [18S-28S rDNA, 5S rDNA and telomeric (TTAGGG)_n]. DAPI staining showed that AT-rich regions were located on the centromere of almost all chromosomes and interstitial banding was not observed. FISH showed that 18S-28S rDNA spread over the short arms of two subtelocentric chromosome pairs and 5S rDNA was located onthe long arm of one subtelocentric chromosome pair. Sequential FISH demonstrated that 18S-28S and 5S rDNA were located on different chromosomes. FISH also showed that the vertebrate telomeric sequence (TTAGGG)_n was located on both ends of each chromosome and no interstitial signals were detected. Sequential 18S-28S rDNA and (TTAGGG)_n FISH indicated that repeated units of the two multicopy families were closely associated on the same chromosome pair.
机译：使用三个重复的DNA探针[18S-28S rDNA，5S rDNA和端粒（TTAGGG）_n]，通过Giemsa染色，DAPI染色和荧光原位杂交（FISH）对Patinopectenyessoensis（Jay，1857）的染色体组进行了表征。 DAPI染色显示，AT丰富的区域位于几乎所有染色体的着丝粒上，并且未观察到间隙带。 FISH表明18S-28S rDNA分布在两个亚远心染色体对的短臂上，而5S rDNA位于一个亚远心染色体对的长臂上。连续FISH证明18S-28S和5S rDNA位于不同的染色体上。 FISH还显示脊椎动物端粒序列（TTAGGG）_n位于每个染色体的两端，并且未检测到间质信号。序列18S-28S rDNA和（TTAGGG）_n FISH表明，两个多拷贝家族的重复单元在同一染色体对上紧密相关。

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