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首页> 外文期刊>Journal of Molecular Neuroscience: MN >Retinal and Optic Nerve Damage is Associated with Early Glial Responses in an Experimental Autoimmune Glaucoma Model
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Retinal and Optic Nerve Damage is Associated with Early Glial Responses in an Experimental Autoimmune Glaucoma Model

机译：视网膜和视神经损伤与实验性自身免疫性青光眼模型中的早期神经胶质反应有关。

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It is well established that the immunization with ocular antigens causes a retinal ganglion cell (RGC) decline, which is accompanied by glia alterations. In this study, the degenerative effects of the immunization with an optic nerve homogenate (ONA) and its purified compound S100 were analyzed on retinas and optic nerves. Since a participation of glia cells in cell death mechanisms is currently discussed, rats were immunized with S100 or ONA. At 14 and 28 days, immune-histological and Western blot analyses were performed to investigate the optic nerve structure (SMI-32), retinal ganglion cells (Brn-3a), apoptosis (cleaved caspase 3, FasL), and glial profile (Iba1, ED1, GFAP, vimentin). Neurofilament dissolution in S100 animals was evident at 14 days (p = 0.047) and increased at 28 days (p = 0.01). ONA optic nerves remained intact at early stages and degenerated later on (p = 0.002). In both groups, RGC loss was detected via immune-histology and Western blot at 28 days (ONA: p = 0.02; S100: p = 0.005). Additionally, more Iba1(+) retinal microglia could be detected at early stages (ONA: p = 0.006; S100: p = 0.028). A slight astrocyte response was detected on Western blots only on ONA retinas (p = 0.01). Hence, the RGC and optic nerve decline was partly antigen dependent, while neuronal loss is paralleled by an early microglial response.
机译：众所周知，用眼抗原进行免疫会引起视网膜神经节细胞（RGC）下降，并伴有神经胶质细胞改变。在这项研究中，分析了视神经匀浆（ONA）及其纯化的化合物S100免疫在视网膜和视神经上的退化作用。由于目前正在讨论神经胶质细胞参与细胞死亡机制，因此用S100或ONA免疫大鼠。在第14天和第28天，进行了免疫组织学和蛋白质印迹分析，以研究视神经结构（SMI-32），视网膜神经节细胞（Brn-3a），细胞凋亡（裂解的半胱天冬酶3，FasL）和神经胶质分布（Iba1） ，ED1，GFAP，波形蛋白）。 S100动物的神经丝溶解在14天时明显（p = 0.047），在28天时增加（p = 0.01）。 ONA视神经在早期仍保持完好无损，随后退化（p = 0.002）。两组均在28天时通过免疫组织学和Western印迹检测到RGC丢失（ONA：p = 0.02; S100：p = 0.005）。此外，在早期可以检测到更多的Iba1（+）视网膜小胶质细胞（ONA：p = 0.006; S100：p = 0.028）。仅在ONA视网膜上通过Western印迹检测到轻微的星形胶质细胞反应（p = 0.01）。因此，RGC和视神经下降部分是抗原依赖性的，而神经元的丧失与早期的小神经胶质反应平行。

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